Ryan P J, Davis M A, Melchior D L
Biochim Biophys Acta. 1983 Mar 15;756(1):106-10. doi: 10.1016/0304-4165(83)90029-6.
Unilamellar phospholipid vesicles loaded with the water-soluble, ionic X-ray contrast agent diatrizoate (Hypaque, Renografin) were manufactured by reverse-phase evaporation for use as organ-enhancing agents in X-ray computed tomography. Encapsulation efficiency was determined as a function of various diatrizoate concentrations in vesicles of varying lipid composition. Loss of encapsulated diatrizoate over 24 h was examined in vesicles composed of several egg phosphatidylcholine/cholesterol ratios. Size estimates for loaded vesicles were obtained by negative-stain electron microscopy, Millipore filtration and light microscopy. Intravenous in vivo injection of loaded vesicles in the rat resulted in significant enhancement of both spleen and liver on subsequent scans. Vesicles were similarly prepared with the water-soluble, nonionic agent metrizamide (Amipaque). Encapsulation efficiency was determined, and in vivo behavior was observed.
通过反相蒸发法制备了负载水溶性离子型X射线造影剂泛影葡胺（Hypaque、Renografin）的单层磷脂囊泡，用作X射线计算机断层扫描中的器官增强剂。在不同脂质组成的囊泡中，测定了包封效率与各种泛影葡胺浓度的函数关系。在由几种卵磷脂/胆固醇比例组成的囊泡中，检测了24小时内包封的泛影葡胺的损失情况。通过负染电子显微镜、密理博过滤和光学显微镜对负载囊泡进行了尺寸估算。在大鼠体内静脉注射负载囊泡后，后续扫描显示脾脏和肝脏均有显著增强。用水溶性非离子型药物甲泛葡胺（Amipaque）制备了类似的囊泡。测定了包封效率，并观察了其体内行为。
