一种刺激可渗透动物细胞中DNA复制的可溶性因子的亚细胞定位。

Subcellular location of a soluble factor that stimulates DNA replication in permeable animal cells.

作者信息

Myers C A, Patel P I, Miller M R

出版信息

Exp Cell Res. 1983 Jan;143(1):227-36. doi: 10.1016/0014-4827(83)90123-4.

Abstract

The subcellular location of a soluble DNA replication-stimulating factor released from lysolecithin-permeabilized cultured mammalian cells was investigated by cytochalasin B (CB) enucleation. In both Chinese hamster ovary cells and baby hamster kidney (BHK) cells, approx. 70% of the total activity of the replication-stimulating factor was associated with the karyoplast fraction. In neither cell line did the replication-stimulating factor show any significant change in activity or in subcellular location between G1 and S period synchronized cells. The general nature and mechanism of action of this stimulatory factor were characterized in order to show that it was similar to "cytoplasmic" factors reported to stimulate DNA replication in other subcellular systems. The predominantly nuclear location of the replication-stimulating factor supports a physiological role in DNA metabolism in animal cells.

摘要

通过细胞松弛素B（CB）去核法，研究了从溶血卵磷脂通透处理的培养哺乳动物细胞中释放的可溶性DNA复制刺激因子的亚细胞定位。在中国仓鼠卵巢细胞和幼仓鼠肾（BHK）细胞中，复制刺激因子的总活性约70%与核体部分相关。在这两种细胞系中，G1期和S期同步化细胞之间的复制刺激因子在活性或亚细胞定位上均未显示出任何显著变化。对这种刺激因子的一般性质和作用机制进行了表征，以表明它类似于其他亚细胞系统中报道的刺激DNA复制的“细胞质”因子。复制刺激因子主要位于细胞核，这支持了其在动物细胞DNA代谢中的生理作用。