Carneiro L H, Hassón-Voloch A
Int J Biochem. 1983;15(1):111-4. doi: 10.1016/0020-711x(83)90018-6.
Creatine kinase (CPK) isozymes of extracts from the electric organ, dorsal muscle and brain of Electrophorus electricus (L.) were analysed with Cellogel electrophoresis. A single component corresponding to the MB-form was obtained for both electric organ and the dorsal muscle. The BB-form was present in the brain extract. 2. Upon acetone fractionation of the aqueous of electric organ, the final fraction was submitted to gel filtration and presented a single peak of CPK activity. 3. Characterization of this fraction by thin-layer gel filtration indicated an apparent molecular weight of 80,000 which corresponds to the enzyme dimeric structure. 4. The implications of this finding with the muscular origin of the electric organ are discussed.
用电泳法分析了电鳗（Electrophorus electricus (L.)）的电器官、背肌和脑提取物中的肌酸激酶（CPK）同工酶。电器官和背肌均获得了对应于MB型的单一成分。BB型存在于脑提取物中。2. 对电器官的水性提取物进行丙酮分级分离后，将最终级分进行凝胶过滤，呈现出单一的CPK活性峰。3. 通过薄层凝胶过滤对该级分进行表征，表明其表观分子量为80,000，这与酶的二聚体结构相对应。4. 讨论了这一发现与电器官肌肉起源的关系。
