牛肉肝谷氨酸脱氢酶：胰凝乳蛋白酶部分蛋白酶解的影响
Beef liver glutamate dehydrogenase: effects of partial proteolysis with chymotrypsin.
作者信息
Aitchison M J, Engel P C
出版信息
Int J Biochem. 1983;15(1):79-85. doi: 10.1016/0020-711x(83)90014-9.
PMID:6825906
Abstract
- After chymotryptic digestion of bovine glutamate dehydrogenase, the assay conditions determine whether activation or inhibition is observed. 2. The major fragments appear to remain physically associated. 3. Responses to both GTP and ADP are altered. Inhibition by GTP at pH 7 and 8 is almost abolished. 4. Out of various ligand combinations tested, GTP and NADH together provide the best protection against all the proteolytic effects.
摘要
- 用胰凝乳蛋白酶消化牛谷氨酸脱氢酶后，测定条件决定观察到的是激活还是抑制。2. 主要片段似乎在物理上保持关联。3. 对GTP和ADP的反应均发生改变。在pH 7和8时，GTP的抑制作用几乎消失。4. 在测试的各种配体组合中，GTP和NADH共同提供了针对所有蛋白水解作用的最佳保护。
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