Decker S J, Kinsey W H
Dev Biol. 1983 Mar;96(1):37-45. doi: 10.1016/0012-1606(83)90309-3.
In order to evaluate the potential role of cortical vesicle exocytosis in modifying the egg surface at fertilization, we have begun characterization of the cortical secretory vesicle. Earlier reports (N. K. Detering, G. L. Decker, E. D. Schmell, and W. Lennarz, 1977, J. Cell Biol. 75, 899-914) have described the isolation of an egg cell surface complex which consists of the egg plasma membrane and cortical secretory vesicles. We have now developed a method of dissassembling the cell surface complex and isolating the cortical vesicles. The very low levels of contaminating plasma membrane in this preparation allow the meaningful comparison of plasma membrane and cortical vesicle proteins and lipids. The cortical vesicles were found to be rich in high-molecular-weight PAS-positive proteins. The majority of these glycoconjugates were solubilized by hypotonic lysis of the cortical vesicles and probably represent proteins sequestered inside the intact vesicles. The fatty acid composition of the cortical vesicles was found to be unusually high in arachidonic acid. The fatty acid composition of the cortical vesicles was closely similar to that of the plasma membrane; however, the cortical vesicles were substantially higher in cholesterol content.
为了评估皮质囊泡胞吐作用在受精时修饰卵表面的潜在作用，我们已开始对皮质分泌囊泡进行特性分析。早期报告（N. K. 德特林、G. L. 德克尔、E. D. 施梅尔和W. 伦纳兹，1977年，《细胞生物学杂志》75卷，899 - 914页）描述了一种卵细胞表面复合物的分离，该复合物由卵质膜和皮质分泌囊泡组成。我们现在已开发出一种分解细胞表面复合物并分离皮质囊泡的方法。此制备物中极低水平的污染质膜使得能够对质膜和皮质囊泡的蛋白质及脂质进行有意义的比较。发现皮质囊泡富含高分子量的PAS阳性蛋白。这些糖缀合物中的大多数通过皮质囊泡的低渗裂解而溶解，可能代表完整囊泡内隔离的蛋白质。发现皮质囊泡的脂肪酸组成中花生四烯酸含量异常高。皮质囊泡的脂肪酸组成与质膜的非常相似；然而，皮质囊泡的胆固醇含量显著更高。
