Vilim A B, Larocque L
J Assoc Off Anal Chem. 1983 Jan;66(1):176-9.
A cylinder plate microbiological method was developed for the rapid, quantitative determination of penicillin G, ampicillin, and cephapirin in animal tissues. The method uses agar plates seeded with stable spores of Bacillus stearothermophilus var. calidolactis and incubated 4 h at 64 degrees C. Standard curves were obtained for the following ranges of concentration of antibiotics in tissues: 0.02-0.32 IU penicillin G/g, 0.0125-0.2 micrograms ampicillin/g, and 0.02-0.32 micrograms cephapirin/g. The proposed method is suitable not only for penicillin residue analysis, for which the sensitivity has been greatly improved compared with the Sarcina lutea method, but also for depletion studies on these antibiotics, which are commonly used to treat diseases in food-producing animals.
已开发出一种圆柱平板微生物学方法，用于快速、定量测定动物组织中的青霉素G、氨苄青霉素和头孢匹林。该方法使用接种嗜热脂肪芽孢杆菌嗜热变种稳定孢子的琼脂平板，并在64℃下孵育4小时。获得了以下组织中抗生素浓度范围的标准曲线：青霉素G 0.02 - 0.32国际单位/克、氨苄青霉素0.0125 - 0.2微克/克和头孢匹林0.02 - 0.32微克/克。所提出的方法不仅适用于青霉素残留分析，与藤黄八叠球菌法相比，其灵敏度有了很大提高，而且适用于这些常用于治疗食用动物疾病的抗生素的消除研究。
