Uehara S, Soh K, Hoshiai H, Yajima A, Suzuki M, Abe H
Nihon Sanka Fujinka Gakkai Zasshi. 1983 Jan;35(1):19-26.
Ascitic fluid was obtained from the patient of ovarian endometrioid cancer. Collected cells were incubated with Eagle's MEM containing 15% fetal calf serum at 37 degrees C under humidified 5% CO2 and 95% air. The epithelial colonies grew rapidly and were released without fibroblast cells. After the first passage, the cells are growing without interruption for over one year and 35 transfer generations. This cell line has following charactors: 1) The monolayer cultured cells appeared epithelial, pavement like arrangement and piling up, without contact inhibition. 2) In the cytoplasma PAS positive substance can be seen. 3) Desmosome-like structure, gap junction, microvilli and well developed cell organelles can be found by electron microscopy. 4) Chromosomal number shows pseudodiploidy which mode is 47. A submetacentric chromosome was present in all karyotype and identified by G-banding. It consists of No. 11 and a part of No. 1. 5) By heterotransplantation to the nude athymic mouse, the tumor easily develops. 6) By estradiol and promegestone its growth was inhibited. 7) Estrogen and progesterone receptors were not detected in the cytosol.
腹水取自卵巢子宫内膜样癌患者。收集的细胞在含有15%胎牛血清的伊格尔氏基本培养基中，于37℃、5%二氧化碳和95%空气的湿润环境下培养。上皮细胞集落生长迅速，且在无成纤维细胞的情况下释放出来。首次传代后，细胞持续生长超过一年，传代35次。该细胞系具有以下特征：1）单层培养的细胞呈上皮样，排列成铺路石状且有堆积现象，无接触抑制。2）细胞质中可见PAS阳性物质。3）通过电子显微镜可发现桥粒样结构、缝隙连接、微绒毛以及发育良好的细胞器。4）染色体数目显示为假二倍体，众数为47。所有核型中均存在一条亚中着丝粒染色体，经G带分析鉴定，它由11号染色体和1号染色体的一部分组成。5）通过异种移植到无胸腺裸鼠体内，肿瘤易于形成。6）雌二醇和醋酸甲羟孕酮可抑制其生长。7）在细胞溶质中未检测到雌激素和孕激素受体。
