Chase R, Kamil R
J Neurobiol. 1983 Jan;14(1):43-50. doi: 10.1002/neu.480140106.
This study examined certain structural and functional aspects of the olfactory system in regenerated posterior tentacles of the terrestrial snail Achatina fulica. Regeneration of the epithelial sensory pad occurs with accurate size regulation. All five neuronal cell types which are normally revealed by horseradish peroxidase backfilling are also regenerated. The sensory cells attain normal numbers at about 20 weeks postlesion. The organization of neuronal elements within the tentacle is chaotic, however, at early stages of regeneration. Even later, the digitlike extensions of the ganglion, which are characteristic of intact tentacles, fail to appear. The recovery of odor sensitivity was evaluated using a tentacular olfactormeter and a behavioral assay that involved locomotor orientation towards the odor stimulus. Thresholds and concentration-dependent response rates were equivalent for regenerated and intact tentacles, tested in the same animals, at 10 weeks post-lesion.
本研究考察了非洲大蜗牛再生后触角嗅觉系统的某些结构和功能方面。上皮感觉垫的再生伴随着精确的尺寸调节。辣根过氧化物酶逆行填充法通常揭示的所有五种神经元细胞类型也会再生。感觉细胞在损伤后约20周达到正常数量。然而，在再生早期，触角内神经元元件的组织是混乱的。即使在后期，完整触角特有的神经节指状延伸也未出现。使用触角嗅觉计和涉及向气味刺激进行运动定向的行为测定法评估气味敏感性的恢复情况。在损伤后10周，对同一动物体内再生触角和完整触角进行测试，其阈值和浓度依赖性反应率相当。
