Gatlin D M, Wilson R P
J Nutr. 1983 Mar;113(3):630-5. doi: 10.1093/jn/113.3.630.
Two experiments were conducted in order to establish the essentiality of zinc for fingerling channel catfish (Ictalurus punctatus) and to determine their dietary zinc requirement. Purified egg white diets with and without supplemental zinc were fed to channel catfish fingerlings for 12 weeks in the essentiality experiment. Catfish fed the basal diet containing 1.0 - 1.5 mg Zn/kg diet developed deficiency signs such as anorexia and suppressed growth. After week 8 of this experiment, two groups of fish fed the basal diet were converted to the zinc-supplemented diet, which contained 28 mg Zn/kg diet. Deficiency signs in these fish were alleviated after consumption of the zinc-supplemented diet. In the requirement experiment, graded levels of a zinc premix were added to egg white diets and fed to fingerling channel catfish for 14 weeks. Serum alkaline phosphatase, serum zinc, bone zinc and bone calcium data indicated that the minimum zinc requirement of fingerling channel catfish was 20 mg Zn/kg dry diet.
为确定锌对斑点叉尾鮰鱼苗的必要性并测定其日粮锌需求量,进行了两项实验。在必要性实验中,将添加和未添加锌的纯化蛋清日粮投喂给斑点叉尾鮰鱼苗12周。饲喂含1.0 - 1.5毫克锌/千克日粮的基础日粮的鲶鱼出现了诸如厌食和生长受抑制等缺乏症状。在该实验的第8周后,两组饲喂基础日粮的鱼改喂含28毫克锌/千克日粮的补锌日粮。这些鱼在食用补锌日粮后缺乏症状得到缓解。在需求量实验中,向蛋清日粮中添加不同梯度水平的锌预混料,并投喂给斑点叉尾鮰鱼苗14周。血清碱性磷酸酶、血清锌、骨锌和骨钙数据表明,斑点叉尾鮰鱼苗的最低锌需求量为20毫克锌/千克干日粮。