Robinson E H, Lovell R T
J Nutr. 1978 Oct;108(10):1600-5. doi: 10.1093/jn/108.10.1600.
A 3 X 2 factorial feeding study was conducted with channel catfish (Ictalurus punctatus) to evaluate effects of biotin, no biotin, or a biotin antagonist (avidin) in lipid and lipid-free diets. At 10 weeks, fish fed diets containing lipid were significantly larger than fish fed lipid-free diets. At 20 weeks, fish fed diets containing avidin had grown significantly less than those fed the other diets. At 22 weeks, fish fed the lipid diet supplemented with biotin had grown significantly more than those fed the lipid diet without biotin. Fish fed the lipid diet with avidin were found to be anemic and exhibited a marked depigmentation of the skin. Fish fed biotin in lipid and lipid-free diets had higher liver pyruvate carboxylase activity than fish fed diets without supplemental biotin. These results indicate that channel catfish require an exogenous source of biotin for maximum rates of growth and lipid utilization.
对斑点叉尾鮰进行了一项3×2析因饲养研究，以评估在含脂和无脂日粮中添加生物素、不添加生物素或添加生物素拮抗剂（抗生物素蛋白）的效果。在10周时，采食含脂日粮的鱼明显大于采食无脂日粮的鱼。在20周时，采食含抗生物素蛋白日粮的鱼生长显著低于采食其他日粮的鱼。在22周时，采食添加生物素的含脂日粮的鱼生长显著高于采食不含生物素的含脂日粮的鱼。发现采食含抗生物素蛋白的含脂日粮的鱼出现贫血，皮肤明显色素脱失。在含脂和无脂日粮中采食生物素的鱼比采食不添加生物素日粮的鱼具有更高的肝脏丙酮酸羧化酶活性。这些结果表明，斑点叉尾鮰需要外源生物素以实现最大生长率和脂质利用率。
