通过Tb3 +荧光增强和电二色性证明的DNA-Pt复合物的变性水平。

Denaturation level of DNA-Pt complexes evidenced by Tb3+ fluorescence enhancement and electric dichroism.

作者信息

Houssier C, Maquet M N, Fredericq E

出版信息

Biochim Biophys Acta. 1983 Apr 15;739(3):312-6. doi: 10.1016/0167-4781(83)90106-9.

Abstract

The Tb3+ fluorescence is greatly enhanced, as a result of binding of various platinum coordination complexes to DNA, as compared to native DNA. The largest enhancement is observed for cis-Pt(NH3)2Cl2 but the fluorescence intensity does not however reach the level attained for thermally denatured DNA. Diethylenetriamine-Pt(II) produces very little increase of Tb3+ fluorescence. The electric dichroism in the DNA absorption band drastically decreases upon binding of the various Pt compounds investigated except diethylenetriamine-Pt. The results are discussed in terms of the various modes of binding of Pt derivatives to DNA, particularly in relation to the level of denaturation of the double helix.

摘要

与天然DNA相比，由于各种铂配位络合物与DNA结合，Tb3+荧光大大增强。顺式Pt(NH3)2Cl2的荧光增强最大，但荧光强度未达到热变性DNA的水平。二亚乙基三胺-Pt(II)对Tb3+荧光的增加作用很小。除二亚乙基三胺-Pt外，所研究的各种铂化合物与DNA结合后，DNA吸收带中的电二色性急剧降低。根据铂衍生物与DNA的各种结合模式，特别是与双螺旋变性程度的关系，对结果进行了讨论。