Bronstein A D, Leleiko N S, Munro H N
Biochim Biophys Acta. 1983 Apr 15;739(3):334-43. doi: 10.1016/0167-4781(83)90109-4.
Rat intestinal mucosa was separated by eversion and vibration to provide a sequence of fractions from predominantly villus cells to predominantly crypt cells. The proportions of these cell types in each fraction were computed from the concentrations of alkaline phosphatase (villus cells) and thymidine kinase (crypt cells) in each population. The isolated mucosal fractions varied from about 90% villus cells to 90% crypt cells. Following injection of the rats with [3H]thymidine, the nuclei were isolated from each mucosal cell fraction and the amount of radioactivity incorporated into DNA was measured as an index of crypt cell abundance. The isolated nuclei were also incubated with ribonucleoside triphosphates and the amount of RNA synthesized was measured. Nuclei labeled with [3H]thymidine were found only in fractions rich in crypt cells, whereas capacity for RNA synthesis remained very active in mucosal fractions consisting predominantly of villus cells. It is concluded that non-dividing villus cells continue to make RNA.
通过外翻和振荡分离大鼠肠黏膜，以提供一系列从主要为绒毛细胞到主要为隐窝细胞的组分。根据每个群体中碱性磷酸酶（绒毛细胞）和胸苷激酶（隐窝细胞）的浓度计算各组分中这些细胞类型的比例。分离出的黏膜组分从约90%的绒毛细胞到90%的隐窝细胞不等。给大鼠注射[3H]胸苷后，从每个黏膜细胞组分中分离细胞核，并测量掺入DNA中的放射性量，作为隐窝细胞丰度的指标。分离出的细胞核还与核糖核苷三磷酸一起孵育，并测量合成的RNA量。仅在富含隐窝细胞的组分中发现用[3H]胸苷标记的细胞核，而在主要由绒毛细胞组成的黏膜组分中，RNA合成能力仍然非常活跃。结论是非分裂的绒毛细胞继续合成RNA。
