大鼠组织从三磷酸二氢新蝶呤合成生物蝶呤。

Synthesis of biopterin from dihydroneopterin triphosphate by rat tissues.

作者信息

Yoshioka S I, Masada M, Yoshida T, Inoue K, Mizokami T, Akino M

出版信息

Biochim Biophys Acta. 1983 Apr 20;756(3):279-85. doi: 10.1016/0304-4165(83)90336-7.

Abstract

High performance liquid chromatography procedure for the analysis of pterins of biopterin synthesis from dihydroneopterin triphosphate via sepiapterin in rat tissues has been described. Sepiapterin-synthesizing enzyme 1, which catalyzes in the presence of Mg2+ the conversion of dihydroneopterin triphosphate to an intermediate designated compound X was assayed by determining pterin which is formed from compound X under acidic conditions. Sepiapterin- and biopterin-synthesizing activity were also assayed by determining sepiapterin and biopterin, respectively. Analytical results revealed the presence of these activities in most rat tissues examined and high levels were found in kidney, pineal gland and liver. Activities were also detectable in peripheral erythrocytes.

摘要

已描述了一种高效液相色谱法，用于分析大鼠组织中通过蝶哌啶从三磷酸二氢新蝶呤合成生物蝶呤的蝶呤。在Mg2+存在下催化三磷酸二氢新蝶呤转化为中间产物X（称为化合物X）的蝶哌啶合成酶1，通过测定在酸性条件下由化合物X形成的蝶呤来进行测定。还分别通过测定蝶哌啶和生物蝶呤来测定蝶哌啶和生物蝶呤的合成活性。分析结果显示在所检查的大多数大鼠组织中存在这些活性，并且在肾脏、松果体和肝脏中发现了高水平的活性。在外周红细胞中也可检测到活性。