Herz F, Halwer M
Biochim Biophys Acta. 1983 Apr 5;762(2):289-94. doi: 10.1016/0167-4889(83)90083-6.
SW-620, a continuous cell line derived from a poorly differentiated human colon carcinoma, produces two alkaline phosphatases. Under basal conditions the heat-stable, term-placental is the major isoenzyme and the heat-labile, liver/bone/kidney form represents a minor component. Exposing SW-620 cells to sodium butyrate causes induction of increased levels of activity accompanied by a striking shift in isoenzyme distribution not observed heretofore. The activity increase is accounted for entirely by augmentation of the liver/bone/kidney isoenzyme, with the term-placental form not being affected. Two other known alkaline phosphatase inducers, prednisolone and hyperosmolality, do not influence specific activity and isoenzyme distribution. The preferential induction of the liver/bone/kidney form of alkaline phosphatase in SW-620 cells may reflect a butyrate-elicited expression of a more differentiated state.
SW - 620是一种源自低分化人结肠癌的连续细胞系，可产生两种碱性磷酸酶。在基础条件下，热稳定的胎盘型是主要的同工酶，而热不稳定的肝/骨/肾型则是次要成分。将SW - 620细胞暴露于丁酸钠会导致活性水平升高，并伴随着同工酶分布的显著变化，这是迄今为止尚未观察到的。活性增加完全是由肝/骨/肾同工酶的增加引起的，胎盘型不受影响。另外两种已知的碱性磷酸酶诱导剂，泼尼松龙和高渗状态，不影响比活性和同工酶分布。SW - 620细胞中肝/骨/肾型碱性磷酸酶的优先诱导可能反映了丁酸盐引发的更分化状态的表达。
