Fukushima K, Sasaki I, Hasegawa H, Takahashi K, Naito H, Funayama Y, Matsuno S
First Department of Surgery, Tokai University School of Medicine, Sendai, Japan.
Dig Dis Sci. 1998 May;43(5):1116-23. doi: 10.1023/a:1018807524075.
Sodium butyrate is a well-recognized differentiating agent inducing alkaline phosphatase activity, one of the epithelial differentiation markers. When IEC6 cells, a nontransformed, small intestinal epithelial cell line, were cultured with butyrate, this substrate induced alkaline phosphate activity in a time- and dose-dependent fashion. However, the type of isoenzyme involved was a liver-type, not an intestinal-type. Electron microscopy revealed that the induced activity was strictly localized in the cytosol and not on the plasma membrane. However, disaccharidase activities, another kind of differentiation marker, were also enhanced by sodium butyrate. In addition, the positive cells demonstrating the presence of alkaline phosphatase activity were preferentially observed in tubular structures. These data show that butyrate-induced alkaline phosphatase activity is closely associated with differentiation-like phenomena in IEC6 cells, although the type of isoenzyme and cellular localization of the activity are different from those observed in mucosa.
丁酸钠是一种公认的诱导碱性磷酸酶活性的分化剂，碱性磷酸酶是上皮分化标志物之一。当非转化的小肠上皮细胞系IEC6细胞与丁酸盐一起培养时，这种底物以时间和剂量依赖性方式诱导碱性磷酸酶活性。然而，所涉及的同工酶类型是肝型的，而非肠型的。电子显微镜显示，诱导的活性严格定位于细胞质中，而非质膜上。然而，另一种分化标志物双糖酶活性也被丁酸钠增强。此外，在管状结构中优先观察到显示碱性磷酸酶活性的阳性细胞。这些数据表明，丁酸盐诱导的碱性磷酸酶活性与IEC6细胞中的分化样现象密切相关，尽管该活性的同工酶类型和细胞定位与在黏膜中观察到的不同。
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