Ivanov A V, Ganago A O
Biofizika. 1983 Jan-Feb;28(1):45-9.
Low-temperature (77 K) luminescence of pea chloroplasts and sub-chloroplast particles enriched with different components of photosynthetic pigment apparatus, and changes of luminescence induced by ruby laser pulse irradiation (694.3 nm) at various intensities were studied. The measured spectra showed selective laser - induced damage revealed by a predominant decrease of longer - wavelength luminescence band (near 730 nm). The effect is associated with light subchloroplast particles. Damage in PSI reaction centres occurred at power densities higher than a threshold value (5-8) X 10(10) W . m-2. No significant selective changes in luminescence spectra of PS2 pigment forms were observed at power densities up to 10(12) W . m-2. PSI - associated pigment forms that luminescence at 750-760 nm appear to be the most sensitive to laser irradiation. These forms are presumably the "sinks" for extra excitation energy in PSI.
研究了豌豆叶绿体和富含光合色素装置不同组分的亚叶绿体颗粒在低温（77K）下的发光情况，以及红宝石激光脉冲（694.3nm）在不同强度照射下引起的发光变化。测量光谱显示出选择性激光诱导损伤，表现为较长波长发光带（近730nm）显著下降。这种效应与轻质亚叶绿体颗粒有关。当功率密度高于阈值（5 - 8）×10¹⁰W·m⁻²时，PSI反应中心发生损伤。在功率密度高达10¹²W·m⁻²时，未观察到PS2色素形式的发光光谱有明显的选择性变化。在750 - 760nm处发光的与PSI相关的色素形式似乎对激光照射最为敏感。这些形式可能是PSI中额外激发能的“汇集处”。
