Horoszewicz J S, Leong S S, Kawinski E, Karr J P, Rosenthal H, Chu T M, Mirand E A, Murphy G P
Cancer Res. 1983 Apr;43(4):1809-18.
The LNCaP cell line was established from a metastatic lesion of human prostatic adenocarcinoma. The LNCaP cells grow readily in vitro (up to 8 x 10(5) cells/sq cm; doubling time, 60 hr), form clones in semisolid media, are highly resistant to human fibroblast interferon, and show an aneuploid (modal number, 76 to 91) human male karyotype with several marker chromosomes. The malignant properties of LNCaP cells are maintained. Athymic nude mice develop tumors at the injection site (volume-doubling time, 86 hr). Functional differentiation is preserved; both cultures and tumor produce acid phosphatase. High-affinity specific androgen receptors are present in the cytosol and nuclear fractions of cells in culture and in tumors. Estrogen receptors are demonstrable in the cytosol. The model is hormonally responsive. In vitro, 5 alpha-dihydrotestosterone modulates cell growth and stimulates acid phosphatase production. In vivo, the frequency of tumor development and the mean time of tumor appearance are significantly different for either sex. Male mice develop tumors earlier and at a greater frequency than do females. Hormonal manipulations show that, regardless of sex, the frequency of tumor development correlates with serum androgen levels. The rate of the tumor growth, however, is independent of the gender of hormonal status of the host.
LNCaP细胞系源自人前列腺腺癌的转移病灶。LNCaP细胞在体外易于生长（可达8×10⁵个细胞/平方厘米；倍增时间为60小时），在半固体培养基中形成克隆，对人成纤维细胞干扰素具有高度抗性，并显示出具有几条标记染色体的非整倍体（众数为76至91）人类男性核型。LNCaP细胞的恶性特性得以维持。无胸腺裸鼠在注射部位发生肿瘤（体积倍增时间为86小时）。功能分化得以保留；培养物和肿瘤均产生酸性磷酸酶。在培养的细胞和肿瘤的胞质溶胶和细胞核部分中存在高亲和力的特异性雄激素受体。在胞质溶胶中可检测到雌激素受体。该模型具有激素反应性。在体外，5α-二氢睾酮调节细胞生长并刺激酸性磷酸酶的产生。在体内，肿瘤发生的频率和肿瘤出现的平均时间在两性中存在显著差异。雄性小鼠比雌性小鼠更早且更频繁地发生肿瘤。激素操作表明，无论性别如何，肿瘤发生的频率与血清雄激素水平相关。然而，肿瘤生长速率与宿主的性别或激素状态无关。
