Buttery J E, Ludvigsen N, Braiotta E A, Pannall P R
Clin Chem. 1983 Apr;29(4):700-2.
For this direct colorimetry of urinary oxalate, commercially available oxalate oxidase (EC 1.2.3.4) is used. The urine is first diluted, to diminish the effect of interfering substances. Analytical recovery of oxalate from urines with five different oxalate concentrations (0.4 to 2.0 mmol/L) ranged from 92 to 109% (mean 99%). The within-day and between-day precision (CV) of the method for a wide range of oxalate concentrations averaged better than 10%. There is good correlation (r = 0.977) between this enzymatic method (y) and the chemical method of Hodgkinson and Williams (x) [Clin Chim Acta 36: 127-132, 1972], the regression equation being y = 1.014x + 0.061. Urines with added ascorbate give falsely increased results. The proposed method is inexpensive and simple to perform.
对于这种尿草酸的直接比色法，使用市售的草酸氧化酶（EC 1.2.3.4）。首先将尿液稀释，以减少干扰物质的影响。从五种不同草酸浓度（0.4至2.0 mmol/L）的尿液中草酸的分析回收率为92%至109%（平均99%）。该方法在较宽的草酸浓度范围内的日内和日间精密度（CV）平均优于10%。这种酶法（y）与霍奇金森和威廉姆斯的化学法（x）[《临床化学学报》36: 127 - 132, 1972]之间存在良好的相关性（r = 0.977），回归方程为y = 1.014x + 0.061。添加了抗坏血酸的尿液会给出错误升高的结果。所提出的方法成本低廉且操作简单。
