Richter M, Richter M, Sklar S
Clin Invest Med. 1983;6(1):19-24.
The sera of a number of individuals containing anti-A and anti-B antibodies were investigated for their ability to sensitize group AB, Rh positive erythrocytes to lysis by allogeneic circulating cells in the allogeneic ADCC assay. There was no correlation between the hemagglutination titer and the capacity to mediate ADCC lysis. Only IgG, and not IgM, alloantibodies could mediate this cytotoxic activity. A number of the alloantisera facilitated cytotoxic activity by lymphocytes whereas other alloantisera facilitated lysis by monocytes and not lymphocytes. All of the alloantisera tested sensitized the target erythrocytes to lysis by neutrophils. It is concluded that one cannot predict whether the lymphocyte or the monocyte will be the cytotoxic mononuclear cell in the human allogeneic ADCC assay since the alloantibodies, apparently directed toward the presumed identical surface structures on the identical target cells, can nevertheless preferentially impart cytotoxic activity to either of these 2 cells. The mechanism whereby the antibodies determine whether lymphocytes or monocytes will be the cytotoxic cells can only be conjectured upon at the present time.
在同种异体抗体依赖细胞介导的细胞毒性（ADCC）试验中，研究了一些含有抗A和抗B抗体的个体血清使AB型Rh阳性红细胞被同种异体循环细胞溶解的能力。血凝滴度与介导ADCC溶解的能力之间没有相关性。只有IgG同种异体抗体，而不是IgM同种异体抗体，能够介导这种细胞毒性活性。一些同种异体抗血清促进淋巴细胞的细胞毒性活性，而其他同种异体抗血清促进单核细胞而非淋巴细胞的溶解。所有测试的同种异体抗血清都使靶红细胞对中性粒细胞的溶解敏感。得出的结论是，在人类同种异体ADCC试验中，无法预测淋巴细胞还是单核细胞将成为细胞毒性单核细胞，因为同种异体抗体显然针对相同靶细胞上假定相同的表面结构，但仍可优先赋予这两种细胞中的任何一种细胞毒性活性。目前只能推测抗体决定淋巴细胞还是单核细胞将成为细胞毒性细胞的机制。
