Feigenbaum A M, de Groot N, Hochberg A A
Mol Biol Rep. 1978 Jun 16;4(2):111-5. doi: 10.1007/BF00775971.
Rough endoplasmic reticulum membranes were dissolved in 1% deoxycholate and the deoxycholate was then dialysed out for five days. Well-defined bilayer vesicles were formed only if the dialysis was performed at room temperature for the first six hours. The vesicles were separated into a pelleted fraction (Fraction 1) and a fluffy layer (Fraction II) by centrifugation. As measured by amino acid incorporation ability, Fraction II bound polysomes, while Fraction I did not. When smooth endoplasmic reticulum was assembled, it was found that Fraction II so derived had a polysome binding capacity which was more sensitive to increased KCl concentrations (25 mM - 100 mM KCl) and that it bound significantly more monosomes than the corresponding fraction derived from rough membranes. The SDS-polyacrylamide polypeptide patterns of the various fractions were compared.
糙面内质网膜溶解于1%脱氧胆酸盐中，然后将脱氧胆酸盐透析五天。只有在前六个小时在室温下进行透析，才会形成明确的双层囊泡。通过离心将囊泡分离成沉淀部分（部分I）和蓬松层（部分II）。通过氨基酸掺入能力测定，部分II结合多核糖体，而部分I不结合。当组装滑面内质网时，发现由此衍生的部分II具有对增加的KCl浓度（25 mM - 100 mM KCl）更敏感的多核糖体结合能力，并且它结合的单体比来自糙面内质网的相应部分明显更多。比较了各个部分的SDS-聚丙烯酰胺多肽图谱。
