Yamada K M, Critchley D R, Fishman P H, Moss J
Exp Cell Res. 1983 Feb;143(2):295-302. doi: 10.1016/0014-4827(83)90054-x.
The major cell-surface glycoprotein fibronectin mediates a variety of cellular adhesive interactions that have been reported to be competitively inhibited by gangliosides. These effects suggest a possible function of gangliosides as receptors for fibronectin. To test this hypothesis more directly, we examined the interaction of endogenous fibronectin with a ganglioside-deficient cell line, NCTC 2071. These cells, which grow in serum-free medium, synthesized fibronectin. The fibronectin did not bind to these cells, but instead bound diffusely to the culture substratum. When the cells were cultured in medium containing ganglioside, the fibronectin became bound to the cell surface in fibrillar strands. The order of effectiveness of purified gangliosides was GT1b greater than GD1a greater than GM1 greater than GM2 greater than GM3. The effect with mixed gangliosides was accompanied by a restoration of cellular capacity to bind and to respond to cholera toxin. Treatment of the cells with several phospholipids did not alter fibronectin binding. Our results support the hypothesis that gangliosides can help mediate the binding of fibronectin to fibroblasts.
主要的细胞表面糖蛋白纤连蛋白介导多种细胞黏附相互作用，据报道这些相互作用会受到神经节苷脂的竞争性抑制。这些效应表明神经节苷脂可能作为纤连蛋白的受体发挥作用。为了更直接地验证这一假设，我们研究了内源性纤连蛋白与神经节苷脂缺陷细胞系NCTC 2071的相互作用。这些在无血清培养基中生长的细胞能合成纤连蛋白。纤连蛋白不与这些细胞结合，而是分散地结合在培养底物上。当细胞在含有神经节苷脂的培养基中培养时，纤连蛋白以纤维状链的形式结合到细胞表面。纯化神经节苷脂的有效顺序为GT1b＞GD1a＞GM1＞GM2＞GM3。混合神经节苷脂的作用伴随着细胞结合和对霍乱毒素反应能力的恢复。用几种磷脂处理细胞不会改变纤连蛋白的结合。我们的结果支持神经节苷脂可帮助介导纤连蛋白与成纤维细胞结合这一假设。
