酸性小泡与海胆卵对胺的摄取
Acidic vesicles and the uptake of amines by sea urchin eggs.
作者信息
Christen R
出版信息
Exp Cell Res. 1983 Feb;143(2):319-25. doi: 10.1016/0014-4827(83)90057-5.
The radioactive amine [14C]methylamine is accumulated to a great extent by eggs, with kinetics that are dependent upon temperature (Q10 = 5) and sensitive to metabolic inhibitors. Efflux of [14C]methylamine from eggs (preloaded with tracer concentrations) is increased immediately after fertilization or NH4Cl activation. Fluorescent amines (9-aminoacridine (9AA) and acridine orange (AO)) are concentrated in small intracellular compartments, presumably vesicles. The possible role of these vesicles in the accumulation of amines by sea urchin eggs and the activation of the metabolism that ensues is discussed.
放射性胺类物质[¹⁴C]甲胺在很大程度上会被卵子积累，其动力学过程取决于温度（Q₁₀ = 5），并且对代谢抑制剂敏感。受精或氯化铵激活后，[¹⁴C]甲胺从预先加载示踪剂浓度的卵子中的流出会立即增加。荧光胺类物质（9-氨基吖啶（9AA）和吖啶橙（AO））集中在小的细胞内区室中，推测为囊泡。本文讨论了这些囊泡在海胆卵子积累胺类物质以及随后的代谢激活过程中可能发挥的作用。
Zotero 插件