Carbone G, Colombo M P, Sensi M L, Cernuschi A, Parmiani G
Int J Cancer. 1983 Apr 15;31(4):483-9. doi: 10.1002/ijc.2910310414.
In this study we have analyzed the in vitro cell-mediated cytotoxicity of immune peritoneal exudate cells (PEC), elicited in syngeneic mice against the MCA-induced, TSTA-bearing BALB/c fibrosarcoma CA-2, GI-17 and C-3. The 4 h 51Cr-release assay showed the immune PEC effectors to be specifically cytotoxic to fibrosarcoma used for the immunization, but not to other syngeneic MCA-induced tumors or normal fibroblasts. Cold target inhibition experiments on CA-2 cells confirmed the specificity of the reaction. When PEC, lymph-node and spleen cells from BALB/c anti-CA-2 mice were compared for anti-tumor activity, only PEC were found to kill tumor cells significantly. PEC effectors did not have a significant level of NK or NC activities since they were unable to destroy YAC-1 or WEHI-164 tumor cells. PEC anti-CA-2 were analyzed for the expression of T-cell markers by anti-Thy 1.2, anti-Ly 1.2 and Ly 2.2 monoclonal antibodies. Anti-tumor specific effector cells were identified as mature T cells since they were not adherent to plastic and showed Thy 1.2+, Ly 1.2- and Ly 2.2+ phenotypes. In addition, anti-H-2Kd but not anti-H-2Dd alloantiserum added to target cells, blocked CA-2 tumor lysis, thus supporting the conclusion that the T-cell response against TSTA is H-2 restricted.
在本研究中，我们分析了同基因小鼠体内诱发的免疫腹膜渗出细胞（PEC）对甲基胆蒽诱导的、携带肿瘤特异性移植抗原（TSTA）的BALB/c纤维肉瘤CA-2、GI-17和C-3的体外细胞介导细胞毒性。4小时51Cr释放试验表明，免疫PEC效应细胞对用于免疫的纤维肉瘤具有特异性细胞毒性，但对其他同基因甲基胆蒽诱导的肿瘤或正常成纤维细胞则无此作用。对CA-2细胞进行的冷靶抑制实验证实了反应的特异性。当比较BALB/c抗CA-2小鼠的PEC、淋巴结细胞和脾细胞的抗肿瘤活性时，仅发现PEC能显著杀伤肿瘤细胞。PEC效应细胞没有显著水平的自然杀伤（NK）或自然细胞毒性（NC）活性，因为它们无法破坏YAC-1或WEHI-164肿瘤细胞。通过抗Thy 1.2、抗Ly 1.2和Ly 2.2单克隆抗体分析了PEC抗CA-2的T细胞标志物表达。抗肿瘤特异性效应细胞被鉴定为成熟T细胞，因为它们不粘附于塑料，且表现出Thy 1.2+、Ly 1.2-和Ly 2.2+表型。此外，添加到靶细胞的抗H-2Kd同种异型抗血清而非抗H-2Dd同种异型抗血清可阻断CA-2肿瘤溶解，从而支持针对TSTA的T细胞反应受H-2限制这一结论。
