多价细菌荚膜多糖疫苗中多糖分子量多分散性的免疫电泳表征
Immunoelectrophoretic characterization of the molecular weight polydispersion of polysaccharides in multivalent bacterial capsular polysaccharide vaccines.
作者信息
Porro M, Fabbiani S, Marsili I, Viti S, Saletti M
出版信息
J Biol Stand. 1983 Jan;11(1):65-74. doi: 10.1016/s0092-1157(83)80047-x.
The molecular weight polydispersion of single antigens present in multivalent bacterial capsular polysaccharide vaccines has been characterized by an immunoelectrophoretic method. Chromatographic effluents from Sepharose gel of bacterial capsular polysaccharide vaccines were tested by fused-rocket immunoelectrophoresis and the distribution coefficient (Kd) of each polysaccharide present in the mixture was calculated. The method appeared to be efficient and reproducible. However, different Kd values were obtained by immunoelectrophoretic and chemical or physical analysis of the chromatographic effluents of each single polysaccharide component. The use of this immunoelectrophoretic procedure was extended to the potency control of multivalent meningococcal and pneumococcal polysaccharide vaccines in order to detect changes in the molecular weight polydispersion of each antigen with time.
采用免疫电泳法对多价细菌荚膜多糖疫苗中单一抗原的分子量多分散性进行了表征。通过火箭免疫电泳对细菌荚膜多糖疫苗的琼脂糖凝胶色谱流出物进行检测，并计算混合物中每种多糖的分配系数（Kd）。该方法似乎有效且可重复。然而，通过对每种单一多糖成分的色谱流出物进行免疫电泳以及化学或物理分析，得到了不同的Kd值。这种免疫电泳方法被扩展用于多价脑膜炎球菌和肺炎球菌多糖疫苗的效价控制，以检测每种抗原的分子量多分散性随时间的变化。
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