Jones C, Currie F
Chemistry Division, National Institute for Biological Standards and Control, South Mimms, Herts, U.K.
Biologicals. 1991 Jan;19(1):41-7. doi: 10.1016/1045-1056(91)90023-d.
Analysis of polysaccharide components of meningococcal- and pneumococcal vaccines was carried out by proton n.m.r. spectroscopy and gas chromatography. The meningococcal polysaccharides were of high purity but showed differences in degree and position of O-acetylation between manufacturers. The level of contamination of the pneumococcal polysaccharides by C-substance was quantified. These methods provide an alternative to immunological methods for determining serotype and purity.
采用质子核磁共振光谱法和气相色谱法对脑膜炎球菌疫苗和肺炎球菌疫苗的多糖成分进行了分析。脑膜炎球菌多糖纯度很高，但不同生产商生产的产品在O - 乙酰化程度和位置上存在差异。对肺炎球菌多糖被C物质污染的水平进行了定量分析。这些方法为确定血清型和纯度提供了免疫方法之外的替代方法。
