• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
In vitro synthesis and membrane insertion of bovine MP26, an integral protein from lens fiber plasma membrane.牛MP26（一种来自晶状体纤维质膜的内在蛋白）的体外合成与膜插入
J Cell Biol. 1983 Mar;96(3):633-8. doi: 10.1083/jcb.96.3.633.
2
Total poly(A+)-messenger RNA from bovine lens cofractionates with sucrose purified fiber cell plasma membrane.
Exp Eye Res. 1984 Jul;39(1):51-60. doi: 10.1016/0014-4835(84)90114-3.
3
Mechanisms of integration of de novo-synthesized polypeptides into membranes: signal-recognition particle is required for integration into microsomal membranes of calcium ATPase and of lens MP26 but not of cytochrome b5.
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7249-53. doi: 10.1073/pnas.80.23.7249.
4
Preparation, characterization, and localization of antisera against bovine MP26, an integral protein from lens fiber plasma membrane.针对牛MP26（一种晶状体纤维质膜整合蛋白）抗血清的制备、表征及定位
J Cell Biol. 1983 Mar;96(3):625-32. doi: 10.1083/jcb.96.3.625.
5
MP26 messenger RNA sequences in normal and cataractous lens. A molecular probe for abundance and distribution of a fiber cell-specific gene product.正常及白内障晶状体中的MP26信使核糖核酸序列。一种用于纤维细胞特异性基因产物丰度和分布的分子探针。
Invest Ophthalmol Vis Sci. 1988 May;29(5):802-13.
6
A lens intercellular junction protein, MP26, is a phosphoprotein.一种晶状体细胞间连接蛋白MP26是一种磷蛋白。
J Cell Biol. 1986 Apr;102(4):1334-43. doi: 10.1083/jcb.102.4.1334.
7
Glycation of MP26 and MP22 in bovine lens membranes.
Biochem Biophys Res Commun. 1992 Jun 15;185(2):496-504. doi: 10.1016/0006-291x(92)91652-7.
8
MP26, a protein of intercellular junctions in the bovine lens: electrophoretic and chromatographic characterization.MP26，牛晶状体中一种细胞间连接蛋白：电泳和色谱特征分析
Exp Eye Res. 1991 May;52(5):629-39. doi: 10.1016/0014-4835(91)90066-n.
9
Structural studies of lens fiber junction protein MP26 by cyanogen bromide cleavage.用溴化氰裂解对晶状体纤维连接蛋白MP26进行结构研究。
Biochem Biophys Res Commun. 1987 Sep 30;147(3):1013-20. doi: 10.1016/s0006-291x(87)80171-7.
10
Limited proteolysis of gap junction protein is intrinsic in mammalian lens fiber-cell plasma membranes.
Biochem Biophys Res Commun. 1987 Sep 15;147(2):846-53. doi: 10.1016/0006-291x(87)91007-2.

引用本文的文献

1
Crystallin gene expression: Insights from studies of transcriptional bursting.晶体蛋白基因表达：转录爆发研究的新见解。
Exp Eye Res. 2021 Jun;207:108564. doi: 10.1016/j.exer.2021.108564. Epub 2021 Apr 21.
2
Cotranslational Integration of Soybean (Glycine max) Oil Body Membrane Protein Oleosin into Microsomal Membranes.大豆（Glycine max）油体膜蛋白油质蛋白共翻译整合到微粒体膜中。
Plant Physiol. 1993 Mar;101(3):993-998. doi: 10.1104/pp.101.3.993.
3
Biosynthesis and processing of ribophorins in the endoplasmic reticulum.内质网中核糖体结合蛋白的生物合成与加工
J Cell Biol. 1984 Sep;99(3):1076-82. doi: 10.1083/jcb.99.3.1076.
4
Mechanisms of integration of de novo-synthesized polypeptides into membranes: signal-recognition particle is required for integration into microsomal membranes of calcium ATPase and of lens MP26 but not of cytochrome b5.
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7249-53. doi: 10.1073/pnas.80.23.7249.
5
Significance of two desmosome plaque-associated polypeptides of molecular weights 75 000 and 83 000.分子量为75000和83000的两种桥粒斑相关多肽的意义
EMBO J. 1983;2(12):2211-5. doi: 10.1002/j.1460-2075.1983.tb01725.x.
6
Junctions between lens fiber cells are labeled with a monoclonal antibody shown to be specific for MP26.晶状体纤维细胞之间的连接用一种单克隆抗体标记，该抗体已被证明对MP26具有特异性。
J Cell Biol. 1985 Jan;100(1):216-25. doi: 10.1083/jcb.100.1.216.
7
Expression of the gene for main intrinsic polypeptide (MIP): separate spatial distributions of MIP and beta-crystallin gene transcripts in rat lens development.主要内在蛋白（MIP）基因的表达：大鼠晶状体发育过程中MIP和β-晶状体蛋白基因转录本的不同空间分布
J Cell Biol. 1988 Mar;106(3):705-14. doi: 10.1083/jcb.106.3.705.
8
Molecular cloning of cDNA for rat liver gap junction protein.大鼠肝脏间隙连接蛋白cDNA的分子克隆
J Cell Biol. 1986 Jul;103(1):123-34. doi: 10.1083/jcb.103.1.123.
9
Electron microscopic observations of reconstituted proteoliposomes with the purified major intrinsic membrane protein of eye lens fibers.对含有纯化的晶状体纤维主要内在膜蛋白的重组蛋白脂质体的电子显微镜观察。
J Cell Biol. 1987 Oct;105(4):1679-89. doi: 10.1083/jcb.105.4.1679.
10
An intrinsic tonoplast protein of protein storage vacuoles in seeds is structurally related to a bacterial solute transporter (GIpF).种子中蛋白质储存液泡的一种内在液泡膜蛋白在结构上与一种细菌溶质转运蛋白（GIpF）相关。
Plant Cell. 1990 Jun;2(6):525-32. doi: 10.1105/tpc.2.6.525.

本文引用的文献

1
Membrane alterations during cataract development in the Nakano mouse lens.中野小鼠晶状体白内障形成过程中的膜改变。
Invest Ophthalmol Vis Sci. 1980 Jun;19(6):619-29.
2
Translocation of proteins across the endoplasmic reticulum. I. Signal recognition protein (SRP) binds to in-vitro-assembled polysomes synthesizing secretory protein.蛋白质在内质网上的转运。I. 信号识别蛋白（SRP）与体外组装的合成分泌蛋白的多核糖体结合。
J Cell Biol. 1981 Nov;91(2 Pt 1):545-50. doi: 10.1083/jcb.91.2.545.
3
The erythrocyte anion transport protein is contranslationally inserted into microsomes.红细胞阴离子转运蛋白在翻译过程中插入微粒体。
Cell. 1982 Jan;28(1):23-31. doi: 10.1016/0092-8674(82)90371-3.
4
Immunocytochemical localization of the lens main intrinsic polypeptide (MIP26) in communicating junctions.晶状体主要内在多肽（MIP26）在通讯连接中的免疫细胞化学定位。
J Cell Biol. 1982 Jan;92(1):213-20. doi: 10.1083/jcb.92.1.213.
5
Mechanisms for the incorporation of proteins in membranes and organelles.蛋白质整合到膜和细胞器中的机制。
J Cell Biol. 1982 Jan;92(1):1-22. doi: 10.1083/jcb.92.1.1.
6
Synthesis and assembly of membrane and organelle proteins.膜蛋白和细胞器蛋白的合成与组装。
Int Rev Cytol Suppl. 1981;12:247-307. doi: 10.1016/b978-0-12-364373-5.50016-0.
7
Assembly of proteins into membranes.蛋白质组装进入细胞膜。
Science. 1980 Nov 21;210(4472):861-8. doi: 10.1126/science.7001628.
8
Secretion requires a cytoplasmically disposed sulphydryl of the RER membrane.分泌需要粗面内质网（RER）膜上一个位于细胞质中的巯基。
Nature. 1980 Jul 10;286(5769):174-6. doi: 10.1038/286174a0.
9
Intracellular protein topogenesis.细胞内蛋白质拓扑结构生成
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1496-500. doi: 10.1073/pnas.77.3.1496.
10
In vitro synthesis of the major lens membrane protein.晶状体主要膜蛋白的体外合成
Proc Natl Acad Sci U S A. 1980 Feb;77(2):725-9. doi: 10.1073/pnas.77.2.725.

牛MP26（一种来自晶状体纤维质膜的内在蛋白）的体外合成与膜插入

In vitro synthesis and membrane insertion of bovine MP26, an integral protein from lens fiber plasma membrane.

作者信息

Paul D L, Goodenough D A

出版信息

J Cell Biol. 1983 Mar;96(3):633-8. doi: 10.1083/jcb.96.3.633.

DOI:10.1083/jcb.96.3.633
PMID:6833374
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2112420/
Abstract

Synthesis of MP26, the principal protein of lens fiber plasma membranes, was directed in the reticulocyte lysate system by poly A mRNA enriched from whole bovine lens RNA using oligo (dt)-cellulose chromatography. Synthesized MP26 was enriched by immune precipitation. The in vitro-synthesized MP26 had an electrophoretic mobility indistinguishable from that of the native molecule. MP26 showed a cotranslational requirement for dog pancreas microsomes in order for membrane association to occur. Microsome-associated in vitro-synthesized MP26 showed a sensitivity to digestion with chymotrypsin which was similar to the sensitivity of native MP26 in isolated lens fiber plasma membranes, indicating correct insertion of the MP26 into the microsome. Synthesis and membrane insertion of MP26 using N-formyl-[35S]methionyl tRNA as label demonstrated that no proteolytic processing or significant glycosylation accompanied membrane insertion. Chymotryptic cleavage of membrane-inserted, N-formyl-[35S]methionine-labeled MP26 resulted in loss of label, suggesting that the N-terminal of the in vitro-synthesized MP26 faces the cytoplasm.

摘要

晶状体纤维质膜的主要蛋白质MP26的合成，是通过使用寡聚（dT）-纤维素色谱法从全牛晶状体RNA中富集的多聚A mRNA，在网织红细胞裂解物系统中进行指导的。合成的MP26通过免疫沉淀进行富集。体外合成的MP26的电泳迁移率与天然分子的电泳迁移率无法区分。MP26为了发生膜结合，对犬胰腺微粒体有共翻译需求。与微粒体相关的体外合成MP26对胰凝乳蛋白酶消化的敏感性，与分离的晶状体纤维质膜中天然MP26的敏感性相似，表明MP26正确插入了微粒体。使用N-甲酰-[35S]甲硫氨酰tRNA作为标记物对MP26进行合成和膜插入，结果表明膜插入过程中没有蛋白水解加工或显著的糖基化现象。对膜插入的、N-甲酰-[35S]甲硫氨酸标记的MP26进行胰凝乳蛋白酶切割，导致标记物丢失，这表明体外合成的MP26的N末端面向细胞质。