若丹明-肌动蛋白在心肌细胞中的掺入模式和时间进程。
Pattern and time course of rhodamine-actin incorporation in cardiac myocytes.
作者信息
Glacy S D
出版信息
J Cell Biol. 1983 Apr;96(4):1164-7. doi: 10.1083/jcb.96.4.1164.
Abstract
Microinjection of skeletal actin labeled with rhodamine into cultured cardiac myocytes was followed by rapid incorporation of fluorescence into myofibrils of the cells. Myocytes examined as shortly as 5 min postinjection displayed fluorescent bands corresponding to the sarcomeres. By 10 min, distinct alternating wide and narrow bands of fluorescence were observed. The wide bands appeared to correspond to the full breadth of the I-bands, whereas the narrow bands of fluorescence corresponded to the M-lines. This pattern of fluorescence remained essentially unchanged for at least 15 h postinjection. The myofibrils of cardiac myocytes were functional after rhodamine-actin incorporation as judged by their ability to contract. The results of this study suggest that cardiac myofibrils are morphologically stable structures which, nonetheless, exhibit extensive exchange of actin subunits.
摘要
将用罗丹明标记的骨骼肌肌动蛋白显微注射到培养的心肌细胞中后，荧光迅速掺入细胞的肌原纤维中。注射后短至5分钟检查的心肌细胞显示出与肌节相对应的荧光带。到10分钟时，观察到明显交替的宽荧光带和窄荧光带。宽荧光带似乎对应于I带的全宽，而窄荧光带对应于M线。这种荧光模式在注射后至少15小时基本保持不变。根据其收缩能力判断，罗丹明-肌动蛋白掺入后心肌细胞的肌原纤维具有功能。这项研究的结果表明，心肌肌原纤维是形态稳定的结构，尽管如此，它们仍表现出肌动蛋白亚基的广泛交换。
相似文献
1
Pattern and time course of rhodamine-actin incorporation in cardiac myocytes.若丹明-肌动蛋白在心肌细胞中的掺入模式和时间进程。
J Cell Biol. 1983 Apr;96(4):1164-7. doi: 10.1083/jcb.96.4.1164.
2
Exchangeability of actin in cardiac myocytes and fibroblasts as determined by fluorescence photobleaching recovery.通过荧光光漂白恢复测定心肌细胞和成纤维细胞中肌动蛋白的交换性。
Tissue Cell. 1998 Apr;30(2):274-80. doi: 10.1016/s0040-8166(98)80076-1.
3
Incorporation of fluorescently labeled actin and tropomyosin into muscle cells.将荧光标记的肌动蛋白和原肌球蛋白整合到肌肉细胞中。
Cell Differ. 1988 Mar;23(1-2):37-52. doi: 10.1016/0045-6039(88)90035-8.
4
Subcellular distribution of rhodamine-actin microinjected into living fibroblastic cells.注射到活的成纤维细胞中的罗丹明 - 肌动蛋白的亚细胞分布。
J Cell Biol. 1983 Oct;97(4):1207-13. doi: 10.1083/jcb.97.4.1207.
5
Sarcomere structures in the rabbit psoas muscle as revealed by fluorescent analogs of phalloidin.用鬼笔环肽荧光类似物揭示的兔腰大肌肌节结构。
Histochemistry. 1984;81(3):301-4. doi: 10.1007/BF00495644.
6
Dynamics of actin in cardiac myofibrils and fibroblast stress fibers.心肌原纤维和成纤维细胞应力纤维中肌动蛋白的动力学
Cell Struct Funct. 1997 Feb;22(1):59-64. doi: 10.1247/csf.22.59.
7
Myofibrillogenesis in living cells microinjected with fluorescently labeled alpha-actinin.在注射了荧光标记α-辅肌动蛋白的活细胞中的肌原纤维生成。
J Cell Biol. 1986 Jun;102(6):2053-66. doi: 10.1083/jcb.102.6.2053.
8
Formation and alignment of Z lines in living chick myotubes microinjected with rhodamine-labeled alpha-actinin.用罗丹明标记的α-辅肌动蛋白显微注射的活鸡肌管中Z线的形成与排列
J Cell Biol. 1986 Dec;103(6 Pt 1):2163-71. doi: 10.1083/jcb.103.6.2163.
9
Relation of nebulin and connectin (titin) to dynamics of actin in nascent myofibrils of cultured skeletal muscle cells.肌动蛋白结合蛋白和肌联蛋白（肌动蛋白丝极长链蛋白）与培养的骨骼肌细胞新生肌原纤维中肌动蛋白动力学的关系。
Exp Cell Res. 1999 Oct 10;252(1):33-40. doi: 10.1006/excr.1999.4611.
10
Incorporation of microinjected biotin-labelled actin into nascent myofibrils of cardiac myocytes: an immunoelectron microscopic study.微注射生物素标记的肌动蛋白掺入心肌细胞新生肌原纤维的研究：免疫电子显微镜观察
J Muscle Res Cell Motil. 1993 Jun;14(3):292-301. doi: 10.1007/BF00123094.
引用本文的文献
1
CapZ and actin capping dynamics increase in myocytes after a bout of exercise and abates in hours after stimulation ends.运动后肌细胞中的 CapZ 和肌动蛋白加帽动力学增加，刺激结束后数小时内会减弱。
J Appl Physiol (1985). 2013 Jun;114(11):1603-9. doi: 10.1152/japplphysiol.01283.2012. Epub 2013 Mar 14.
2
Dynamic regulation of sarcomeric actin filaments in striated muscle.横纹肌中肌动蛋白丝的动态调节。
Cytoskeleton (Hoboken). 2010 Nov;67(11):677-92. doi: 10.1002/cm.20476.
3
Distinct families of Z-line targeting modules in the COOH-terminal region of nebulin.伴肌动蛋白COOH末端区域中不同的Z线靶向模块家族。
J Cell Biol. 2000 Aug 7;150(3):553-66. doi: 10.1083/jcb.150.3.553.
4
Na+/Pi co-transport alters rapidly cytoskeletal protein polymerization dynamics in opossum kidney cells.钠/磷酸盐共转运迅速改变负鼠肾细胞中的细胞骨架蛋白聚合动力学。
Biochem J. 1996 Apr 1;315 ( Pt 1)(Pt 1):241-7. doi: 10.1042/bj3150241.
5
Characterization, cell-surface expression and ligand-binding properties of different truncated N-terminal extracellular domains of the ionotropic glutamate receptor subunit GluR1.离子型谷氨酸受体亚基GluR1不同截短的N端细胞外结构域的表征、细胞表面表达及配体结合特性
Biochem J. 1996 Apr 1;315 ( Pt 1)(Pt 1):217-25. doi: 10.1042/bj3150217.
6
Incorporation of microinjected biotin-labelled actin into nascent myofibrils of cardiac myocytes: an immunoelectron microscopic study.微注射生物素标记的肌动蛋白掺入心肌细胞新生肌原纤维的研究：免疫电子显微镜观察
J Muscle Res Cell Motil. 1993 Jun;14(3):292-301. doi: 10.1007/BF00123094.
7
Analysis of myofibrillar structure and assembly using fluorescently labeled contractile proteins.使用荧光标记的收缩蛋白分析肌原纤维结构与组装。
J Cell Biol. 1984 Mar;98(3):825-33. doi: 10.1083/jcb.98.3.825.
8
Microinjection of fluorescent tubulin into dividing sea urchin cells.将荧光微管蛋白显微注射到分裂中的海胆细胞中。
J Cell Biol. 1983 Oct;97(4):1249-54. doi: 10.1083/jcb.97.4.1249.
9
Subcellular distribution of rhodamine-actin microinjected into living fibroblastic cells.注射到活的成纤维细胞中的罗丹明 - 肌动蛋白的亚细胞分布。
J Cell Biol. 1983 Oct;97(4):1207-13. doi: 10.1083/jcb.97.4.1207.
10
Identical distribution of fluorescently labeled brain and muscle actins in living cardiac fibroblasts and myocytes.荧光标记的脑肌动蛋白和肌肉肌动蛋白在活的心脏成纤维细胞和心肌细胞中的分布相同。
J Cell Biol. 1985 Jan;100(1):292-6. doi: 10.1083/jcb.100.1.292.
本文引用的文献
1
Mobility of cytoplasmic and membrane-associated actin in living cells.活细胞中细胞质和膜相关肌动蛋白的流动性。
Proc Natl Acad Sci U S A. 1982 Aug;79(15):4660-4. doi: 10.1073/pnas.79.15.4660.
2
Protein turnover in muscle cells as visualized by autoradiography.通过放射自显影观察到的肌肉细胞中的蛋白质周转。
Int Rev Cytol. 1980;67:215-57. doi: 10.1016/s0074-7696(08)62430-4.
3
Immunofluorescence comparisons of anti-actin specificity.抗肌动蛋白特异性的免疫荧光比较。
Eur J Cell Biol. 1982 Aug;28(1):115-21.
4
Preparation and characterization of a new molecular cytochemical probe: 5-iodoacetamidofluorescein-labeled actin.一种新型分子细胞化学探针的制备与表征：5-碘乙酰氨基荧光素标记的肌动蛋白
J Histochem Cytochem. 1980 Nov;28(11):1198-206. doi: 10.1177/28.11.6107318.
5
Probing the dynamic equilibrium of actin polymerization by fluorescence energy transfer.通过荧光能量转移探究肌动蛋白聚合的动态平衡
Cell. 1981 Dec;27(3 Pt 2):429-36. doi: 10.1016/0092-8674(81)90384-6.
6
The synthesis and assembly of myofibrils in embryonic muscle.胚胎肌肉中肌原纤维的合成与组装。
Curr Top Dev Biol. 1970;5:235-80. doi: 10.1016/s0070-2153(08)60057-5.
7
Postnatal muscle fiber assembly: localization of newly synthesized myofibrillar proteins.
Science. 1970 Mar 13;167(3924):1499-501. doi: 10.1126/science.167.3924.1499.
8
9
Coordinated synthesis and degradation of actin and myosin in a variety of myogenic and non-myogenic cells.多种成肌细胞和非成肌细胞中肌动蛋白和肌球蛋白的协同合成与降解。
Exp Cell Res. 1976 Feb;97(2):387-93. doi: 10.1016/0014-4827(76)90630-3.
10
Morphometric and autoradiographic study of developing ventricular and atrial myocardium in fetal rats.胎鼠心室和心房心肌发育的形态计量学与放射自显影研究
Lab Invest. 1975 Dec;33(6):696-705.
Zotero 插件