Gallo V, Aloisi F, Levi G
J Neurochem. 1983 Apr;40(4):939-45. doi: 10.1111/j.1471-4159.1983.tb08077.x.
In previous studies we have shown that the depolarization-induced release of preaccumulated acidic amino acids and newly synthesized glutamate from cerebellar synaptosomal preparations is potentiated by gamma-aminobutyric acid (GABA) agonists through a GABAergic presynaptic mechanism. Here we report a systematic analysis of the ionic requirements of the potentiating effect of muscimol on the high K+-evoked release of D-[3H]aspartate. Our studies show that: Ca2+, Na+, and Mg2+ are not required for muscimol to exert its effect; a depolarizing concentration of K+ is a necessary, but not sufficient, condition to observe the presynaptic effect in question; and a minimal Cl- concentration (50-70 mM) is also required. A possible model based on these findings is proposed.
在先前的研究中，我们已经表明，通过一种γ-氨基丁酸（GABA）能的突触前机制，GABA激动剂可增强去极化诱导的从小脑突触体制剂中预积累的酸性氨基酸和新合成的谷氨酸的释放。在此，我们报告了对蝇蕈醇增强高钾诱发的D-[3H]天冬氨酸释放作用的离子需求的系统分析。我们的研究表明：蝇蕈醇发挥其作用不需要Ca2+、Na+和Mg2+；去极化浓度的K+是观察所述突触前效应的必要但不充分条件；并且还需要最低的Cl-浓度（50 - 70 mM）。基于这些发现提出了一个可能的模型。
