Yamamoto Y, Thau R B
J Reprod Fertil. 1983 Mar;67(2):441-6. doi: 10.1530/jrf.0.0670441.
The method for a rapid bioassay for the neutralizing activities of antichorionic gonadotrophin sera is based on the inhibition of the increase in plasma testosterone concentrations in male mice after the injection of antiserum and human chorionic gonadotrophin (hCG). The antisera were obtained from rhesus monkeys immunized against the beta-subunit of ovine luteinizing hormone (oLH beta). The anti-oLH beta sera neutralized the hCG-induced testosterone stimulation. A dose-response relationship for neutralization was found between 25 and 200 microliters antiserum. Anti-hCG sera raised in human, chimpanzee and rabbit also neutralized the biological activity of hCG. The major advantage of this method is that the single injection of antiserum given before hCG administration leads to the hormone-antibody reaction in vivo.
一种用于快速生物测定抗绒毛膜促性腺激素血清中和活性的方法，是基于在注射抗血清和人绒毛膜促性腺激素（hCG）后，抑制雄性小鼠血浆睾酮浓度的升高。抗血清取自免疫羊促黄体生成素β亚基（oLHβ）的恒河猴。抗oLHβ血清中和了hCG诱导的睾酮刺激。在25至200微升抗血清之间发现了中和的剂量反应关系。人、黑猩猩和兔产生的抗hCG血清也中和了hCG的生物活性。该方法的主要优点是在给予hCG之前单次注射抗血清会导致体内激素-抗体反应。
