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[通过超速离心以及用肝素和钙沉淀法分离的血清脂蛋白的化学发光]

[Chemoluminescence of blood serum lipoproteins isolated by means of ultracentrifugation and precipitation with heparin and calcium].

作者信息

Lopukhin Iu M, Vladimirov Iu A, Molodenkov M N, Klebanov G I, Sergienko V I

出版信息

Vopr Med Khim. 1983 Jan-Feb;29(1):116-20.

PMID:6836949
Abstract

Apo-B containing lipoproteins obtained after precipitation with heparin and calcium as well as very low (LPVLD) and low density lipoproteins (LPLD) produced using preparative scale ultracentrifugation had identical chemoluminescent properties. However, LPVLD are involved only slightly in total chemoluminescence of apo-B containing lipoproteins due to their low concentration in blood serum. In blood serum there is the factor having the quenching effect on the chemoluminescence and also exhibiting the antioxidant activity. Subfraction 3 of high density lipoproteins from patients with atherosclerosis differed from the analogous subfraction of donors by its chemoluminescent properties.

摘要

用肝素和钙沉淀后获得的含载脂蛋白B的脂蛋白,以及用制备规模超速离心法产生的极低密度脂蛋白(LPVLD)和低密度脂蛋白(LPLD)具有相同的化学发光特性。然而,由于LPVLD在血清中的浓度较低,它们在含载脂蛋白B的脂蛋白总化学发光中只起轻微作用。血清中存在对化学发光有猝灭作用且具有抗氧化活性的因子。动脉粥样硬化患者高密度脂蛋白的亚组分3在化学发光特性上与供体的类似亚组分不同。

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Vopr Med Khim. 1983 Jan-Feb;29(1):116-20.
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