Potter J E, Kandutsch A A
Biochem Biophys Res Commun. 1983 Jan 27;110(2):512-8. doi: 10.1016/0006-291x(83)91179-8.
The rates of synthesis and tissue concentrations of dolichol derivatives differ markedly in testes and preputial glands from adult C57BL/6J mice. In testes, the rates of free dolichol, dolichyl acyl esters, and dolichyl phosphate synthesis were approximately 5,000, 4,000 and 2,000 dpm/3h/g, respectively. Comparable rates for preputial glands were 12,000, 60,000 and 0 dpm/3h/g. Thus in testes, dolichyl phosphate represented 15-20% of total dolichol synthesis, whereas no de novo dolichyl phosphate synthesis was detected in preputial glands. In testes, free dolichol was the predominant derivative synthesized; in preputial glands, 80% of dolichol synthesized was esterified to fatty acids. The concentration of total dolichol (free alcohol plus acyl esters) was 160 micrograms/g tissue in testes and 1270 micrograms/g tissue in preputial glands. In both tissues, 85-90% of dolichol was esterified to fatty acids, and no dolichyl phosphate was detected.
成年C57BL/6J小鼠睾丸和包皮腺中多萜醇衍生物的合成速率和组织浓度存在显著差异。在睾丸中，游离多萜醇、多萜醇酰基酯和多萜醇磷酸酯的合成速率分别约为5000、4000和2000 dpm/3h/g。包皮腺的相应速率分别为12000、60000和0 dpm/3h/g。因此，在睾丸中，多萜醇磷酸酯占多萜醇总合成量的15 - 20%，而在包皮腺中未检测到从头合成的多萜醇磷酸酯。在睾丸中，游离多萜醇是合成的主要衍生物；在包皮腺中，合成的多萜醇中有80%被酯化为脂肪酸。睾丸中总多萜醇（游离醇加酰基酯）的浓度为160微克/克组织，包皮腺中为1270微克/克组织。在这两种组织中，85 - 90%的多萜醇被酯化为脂肪酸，且未检测到多萜醇磷酸酯。
