一种具有4-O-甲基-D-葡萄糖醛酸特异性的大豆凝集素。

A soybean lectin having 4-O-methyl-D-glucuronic acid specificity.

作者信息

Dombrink-Kurtzman M A, Dick W E, Burton K A, Cadmus M C, Slodki M E

出版信息

Biochem Biophys Res Commun. 1983 Mar 29;111(3):798-803. doi: 10.1016/0006-291x(83)91369-4.

Abstract

Evidence is presented for the presence of a new lectin activity in soybean seeds [Glycine max (L.) Merrill] that has specificity towards the 4-O-methyl-D-glucurono-L-rhamnan exopolysaccharide produced by certain strains of Rhizobium japonicum. Bacterial agglutination and precipitin reactions revealed the lectin activity in phosphate-buffered saline extracts of seeds of all cultivars tested, including the "lectinless" varieties. Reaction of such extracts with carbohydrate haptens demonstrated that the specificity of the binding was towards 4-O-methyl-D-glucuronic acid, D-glucuronic acid and their methyl glycosides.

摘要

有证据表明大豆种子[大豆（Glycine max (L.) Merrill）]中存在一种新的凝集素活性，它对某些日本根瘤菌菌株产生的4-O-甲基-D-葡糖醛酸-L-鼠李聚糖胞外多糖具有特异性。细菌凝集和沉淀反应揭示了在所有测试品种的种子的磷酸盐缓冲盐水提取物中都存在凝集素活性，包括“无凝集素”品种。这种提取物与碳水化合物半抗原的反应表明，结合的特异性是针对4-O-甲基-D-葡糖醛酸、D-葡糖醛酸及其甲基糖苷。