Hunter G K, Heersche J N, Aubin J E
Biochemistry. 1983 Feb 15;22(4):831-7. doi: 10.1021/bi00273a019.
Three proteoglycan fractions have been isolated from clonal populations of osteoblast-like cells derived from fetal rat calvaria. One of these is secreted into the culture medium, is of apparent Mr 350 000, and has a glycosaminoglycan (GAG) composition of 77% chondroitin sulfate (CS) and 20% dermatan sulfate (DS). The remaining two proteoglycan fractions are associated with the cell layer. One of these has an apparent molecular weight of approximately 250 000 and a GAG composition of 54% CS and 40% DS. Both this species and the secreted proteoglycan have GAG chains of Mr 25 000. The other cell-associated proteoglycan contains heparan sulfate (HS), is solubilized by detergents, and appears to be contaminated with a CS proteoglycan. This HS-containing species may be similar to plasma membrane proteoglycans that have been isolated from several other cell types. Rat calvarial clones also synthesize hyaluronic acid and a number of glycoproteins.
已从源自胎鼠颅骨的成骨细胞样细胞克隆群体中分离出三种蛋白聚糖组分。其中一种分泌到培养基中，表观分子量为350000，糖胺聚糖（GAG）组成为77%硫酸软骨素（CS）和20%硫酸皮肤素（DS）。其余两种蛋白聚糖组分与细胞层相关。其中一种表观分子量约为250000，GAG组成为54% CS和40% DS。该物种和分泌的蛋白聚糖都有分子量为25000的GAG链。另一种与细胞相关的蛋白聚糖含有硫酸乙酰肝素（HS），可被去污剂溶解，似乎被CS蛋白聚糖污染。这种含HS的物种可能类似于从其他几种细胞类型中分离出的质膜蛋白聚糖。大鼠颅骨克隆还合成透明质酸和多种糖蛋白。
