alamethicin诱导的脂质双分子层电流-电压曲线不对称性。
Alamethicin-induced current-voltage curve asymmetry in lipid bilayers.
作者信息
Vodyanoy I, Hall J E, Balasubramanian T M
出版信息
Biophys J. 1983 Apr;42(1):71-82. doi: 10.1016/S0006-3495(83)84370-7.
Abstract
We have examined the causes of the asymmetry of the current-voltage curve induced by addition of alamethicin to one side of a black lipid membrane. We find that the alamethicin-induced current-voltage (I-V) curve has an inherent asymmetry. If it were possible to confine all alamethicin molecules to one side of the membrane, the I-V curve would exhibit a positive branch (voltage being measured with respect to the side of the membrane trans to the alamethicin addition) of steeper logarithmic slope than the negative branch and at a lower absolute value of potential. This condition is not usually realized, however, because alamethicin can leak through the membrane, so that, except at very high alamethicin concentrations and in certain kinds of membranes, the positive branch of the current-voltage curve has the same logarithmic slope as the negative branch and appears to arise from alamethicin which diffuses from the cis to the trans side of the membrane. We develop simple quantitative models for these two cases.
摘要
我们研究了在黑色脂质膜一侧添加短杆菌肽后电流-电压曲线不对称的原因。我们发现，短杆菌肽诱导的电流-电压（I-V）曲线具有内在的不对称性。如果有可能将所有短杆菌肽分子限制在膜的一侧，那么I-V曲线将呈现出一个正分支（相对于添加短杆菌肽一侧的膜的另一侧测量电压），其对数斜率比负分支更陡，且电位绝对值更低。然而，这种情况通常无法实现，因为短杆菌肽可以透过膜泄漏，所以，除了在非常高的短杆菌肽浓度下以及某些类型的膜中，电流-电压曲线的正分支与负分支具有相同的对数斜率，并且似乎是由从膜的顺侧扩散到反侧的短杆菌肽引起的。我们针对这两种情况开发了简单的定量模型。
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