与脂质双分子层中电压依赖性电导相关的短杆菌肽A的偶极矩
Dipole moment of alamethicin as related to voltage-dependent conductance in lipid bilayers.
作者信息
Yantorno R, Takashima S, Mueller P
出版信息
Biophys J. 1982 May;38(2):105-10. doi: 10.1016/S0006-3495(82)84536-0.
Abstract
The dipole moment of alamethicin, which produces voltage-dependent conductance in lipid-bilayer membranes, was measured in mixed solvents of ethanol and dioxane. The value of the dipole moment was found to increase from 40 to 75 DU (Debye units), as the concentration of ethanol increased from 0 (pure dioxane) to 40%. The relaxation frequency of alamethicin also changes from 10 to 40 MHz, depending upon the concentration of ethanol in mixed solvents. The length of alamethicin was calculated by using the relaxation time and was found to range from approximately 40 to 20 A. The dipole moment was independently calculated from voltage-dependent conductance and compared with the measured value. The calculated value was found to be larger than the value of direct measurements, indicating that several alamethicin molecules are required to form a conducting pore and that their dipole moments are oriented parallel to each other.
摘要
在脂质双分子层膜中产生电压依赖性电导的短杆菌肽A的偶极矩，是在乙醇和二氧六环的混合溶剂中测量的。当乙醇浓度从0（纯二氧六环）增加到40%时，发现偶极矩的值从40德拜单位增加到75德拜单位。短杆菌肽A的弛豫频率也根据混合溶剂中乙醇的浓度从10兆赫兹变化到40兆赫兹。通过使用弛豫时间计算出短杆菌肽A的长度，发现其范围约为40到20埃。从电压依赖性电导独立计算出偶极矩，并与测量值进行比较。发现计算值大于直接测量值，这表明形成一个导电孔需要几个短杆菌肽A分子，并且它们的偶极矩相互平行排列。
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