Rukenstein A, Greene L A
Brain Res. 1983 Mar 14;263(1):177-80. doi: 10.1016/0006-8993(83)91218-0.
A quantitative bioassay for nerve growth factor (NGF) has been previously described which employs PC13 rat pheochromocytoma cells. The basis of this assay is that rapid neurite regeneration by PC12 cells pretreated ('primed') with NGF for at least a week is quantitatively related to the amount of NGF present in the culture medium. The present experiments demonstrate that NGF-pretreated PC12 cells retain their priming for over a year when appropriately frozen and stored and that such frozen cells can be employed for the quantitative bioassay of NGF. The major advantage of this assay is that large numbers of cells can be primed at a time (in suspension culture) and stored in aliquots until future use. The newly described bioassay employs the frozen primed cells essentially as reagents and avoids the need to have freshly primed cultures on hand.
先前已描述了一种使用PC13大鼠嗜铬细胞瘤细胞的神经生长因子（NGF）定量生物测定法。该测定法的基础是，用NGF预处理（“致敏”）至少一周的PC12细胞的快速神经突再生与培养基中存在的NGF量呈定量关系。目前的实验表明，经过适当冷冻和储存后，经NGF预处理的PC12细胞可保持其致敏状态超过一年，并且这种冷冻细胞可用于NGF的定量生物测定。该测定法的主要优点是可以一次对大量细胞进行致敏（在悬浮培养中），并分装保存以备将来使用。新描述的生物测定法基本上将冷冻致敏细胞用作试剂，无需手头有新鲜致敏的培养物。
