两种人尿核糖核酸酶的放射免疫测定法：人血清中核糖核酸酶的差异测定

Radioimmunoassays for two types of human urinary ribonucleases: differential determination of ribonucleases in human serum.

作者信息

Kurokawa E, Ogawa M, Kurihara M, Ohta T, Kitahara T, Kosaki G, Iwama M, Ohgi K, Irie M

出版信息

Clin Chim Acta. 1983 Feb 28;128(1):83-93. doi: 10.1016/0009-8981(83)90058-x.

Abstract

We developed radioimmunoassays for two types of human urinary ribonucleases. The assays are sensitive, reproducible and specific. One human urinary ribonuclease (RNase UL) showed strong immunological identity with human pancreatic ribonuclease, and the other ribonuclease (RNase Us) showed strong immuno cross-reactivity with human liver ribonuclease. There was no immuno cross-reactivity between these two urinary ribonucleases. Serum immunoreactive RNase UL was eluted as four peaks on phosphocellulose chromatography, whereas immunoreactive RNase US was eluted as a single peak. Serum content of immunoreactive RNase UL was 354 +/- 105 ng/ml (mean +/- SD) in normal individuals, and that of immunoreactive RNase Us was 15.9 +/- 5.7 ng/ml (mean +/- SD). No correlation was demonstrated between these two ribonuclease contents in serum.

摘要

我们开发了针对两种人尿核糖核酸酶的放射免疫测定法。这些测定法灵敏、可重复且具有特异性。一种人尿核糖核酸酶（RNase UL）与人胰腺核糖核酸酶表现出强烈的免疫同一性，另一种核糖核酸酶（RNase Us）与人肝脏核糖核酸酶表现出强烈的免疫交叉反应性。这两种尿核糖核酸酶之间没有免疫交叉反应性。血清免疫反应性RNase UL在磷酸纤维素色谱上洗脱为四个峰，而免疫反应性RNase US洗脱为一个单峰。正常个体中免疫反应性RNase UL的血清含量为354±105 ng/ml（平均值±标准差），免疫反应性RNase Us的血清含量为15.9±5.7 ng/ml（平均值±标准差）。血清中这两种核糖核酸酶的含量之间未显示出相关性。