人精浆核糖核酸酶。血清、尿液和精浆中交叉反应性酶的免疫定量分析。

Human seminal ribonuclease. Immunological quantitation of cross-reactive enzymes in serum, urine and seminal plasma.

作者信息

Sorrentino S, De Prisco R, Libonati M

机构信息

Dipartimento di Chimica Organica e Biologica, Facoltà di Scienze, Università di Napoli, Italy.

出版信息

Biochim Biophys Acta. 1989 Sep 14;998(1):97-101. doi: 10.1016/0167-4838(89)90125-8.

DOI:10.1016/0167-4838(89)90125-8

PMID:2790056

Abstract

The distribution of secretory-type ribonuclease in human serum, urine and seminal plasma has been studied by immunological measurements. Inhibition of enzyme activity by antibodies against pure human seminal RNAase shows that a cross-reactive enzyme is predominant (90%) in seminal plasma and is a significant component (70-80%) in urine and serum. A competitive binding radioimmunoassay has been developed by using specific antibodies and 125I-labelled RNAase as radioligand. The procedure, very sensitive, reproducible and specific, has been used to determine seminal RNAase levels in seminal plasma samples from 48 healthy individuals (age range, 20-58 years). The mean concentration of the enzyme was found to be 6.6 micrograms/ml (S.D. +/- 1.9).

摘要

通过免疫测定法研究了分泌型核糖核酸酶在人血清、尿液和精浆中的分布情况。抗纯人精浆核糖核酸酶抗体对酶活性的抑制作用表明，一种交叉反应性酶在精浆中占主导地位（90%），在尿液和血清中也是重要成分（70 - 80%）。利用特异性抗体和¹²⁵I标记的核糖核酸酶作为放射性配体，开发了一种竞争性结合放射免疫测定法。该方法非常灵敏、可重复且具有特异性，已用于测定48名健康个体（年龄范围20 - 58岁）精浆样本中的精浆核糖核酸酶水平。发现该酶的平均浓度为6.6微克/毫升（标准差±1.9）。