[大鼠肝脏质膜上胰岛素受体相互作用的研究]
[Study of the insulin-receptor interaction on the plasma membranes of rat liver].
作者信息
Liubarskaia S G
出版信息
Probl Endokrinol (Mosk). 1978 Jul-Aug;24(4):61-4.
PMID:683973
Abstract
The radioreceptor method was applied to the study of specific insulin binding on the plasma membranes of the rat liver. Highly purified plasma membranes were obtained and conditions of their incubation with insulin were worked out for this purpose. Two types of insulin receptors were revealed: the first one with Cass=3.8( +/- 0.4)x10(11)M-1 and binding capacity=0.7( +/- 0.1) pM/mg; the second one--with less relationship of Cass=8( +/- 1)x10(10)M-1 and binding capacity=5( +/- 0.7) pM/mg.
摘要
采用放射受体法研究大鼠肝脏质膜上的特异性胰岛素结合。为此获得了高度纯化的质膜，并确定了其与胰岛素孵育的条件。发现了两种类型的胰岛素受体：第一种的解离常数（Kd）为3.8（±0.4）×10⁻¹¹M⁻¹，结合容量为0.7（±0.1）pM/mg；第二种的解离常数关系较小，为8（±1）×10⁻¹⁰M⁻¹，结合容量为5（±0.7）pM/mg。
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