血浆中地尔硫䓬及其代谢物的高效液相色谱分析
High-performance liquid chromatographic analysis of diltiazem and its metabolite in plasma.
作者信息
Verghese C, Smith M S, Aanonsen L, Pritchett E L, Shand D G
出版信息
J Chromatogr. 1983 Jan 14;272(1):149-55. doi: 10.1016/s0378-4347(00)86111-3.
A rapid, selective and reproducible high-performance liquid chromatographic method for the analysis in plasma of the calcium channel blocking agent, diltiazem, and one of its metabolites, deacetyldiltiazem is described. The method involves extraction with the methyl tert.-butyl ether of the drugs and the internal standard (verapamil), back-extraction into sulphuric acid and reversed-phase chromatography with UV detection. Over a concentration range of 10-1000 ng/ml the average coefficient of variation for diltiazem was 5.4% and for deacetyldiltiazem was 8.3%.
本文描述了一种快速、选择性和可重现的高效液相色谱法，用于分析血浆中的钙通道阻滞剂地尔硫䓬及其代谢产物之一去乙酰地尔硫䓬。该方法包括用甲基叔丁基醚提取药物和内标（维拉帕米），反萃取到硫酸中，并用紫外检测进行反相色谱分析。在地尔硫䓬浓度范围为10 - 1000 ng/ml时，其平均变异系数为5.4%，去乙酰地尔硫䓬的平均变异系数为8.3%。
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