Coates P M, Brown S A, Sonawane B R, Koldovsky O
J Nutr. 1983 May;113(5):1046-50. doi: 10.1093/jn/113.5.1046.
Early exposure to cholesterol-enriched, high fat diets has been reported to affect serum cholesterol levels in adult rats. In this study, we investigated the role of dietary fat alone, without cholesterol, by feeding to pregnant rats (from day 18 of gestation) experimental diets containing either high fat (corn oil), low sucrose (HF) or low fat, high sucrose (HS). After birth, mothers and pups were fed the diets until weaning (30 days), when serum cholesterol levels were the same in both groups of pups. Animals were fed stock diet until 7 months. At that time, half the animals from each original group (HF or HS) were challenged with HF diet for 3 days; the other half were fed stock diet. There was no significant difference in serum cholesterol between HF and HS animals fed stock diet: HF, 73 +/- 7 mg/dl (n = 11); HS, 80 +/- 19 (6); P greater than 0.25. Animals originally fed HF diet significantly raised serum cholesterol in response to late HF challenge (140 +/- 31 mg/dl, n = 10, P less than 0.001 vs. stock fed); the original HS-fed group did not (93 +/- 7 mg/dl, n = 5, P greater than 0.19). Early exposure to HF diet, even without cholesterol, could evoke a hypercholesterolemic response in adulthood following challenge by brief exposure to HF diet.
据报道，成年大鼠早期接触富含胆固醇的高脂肪饮食会影响其血清胆固醇水平。在本研究中，我们通过给怀孕大鼠（从妊娠第18天开始）喂食含有高脂肪（玉米油）、低蔗糖（HF）或低脂肪、高蔗糖（HS）的实验性饮食，来研究不含胆固醇的单纯膳食脂肪的作用。出生后，母鼠和幼鼠继续食用这些饮食直至断奶（30天），此时两组幼鼠的血清胆固醇水平相同。动物继续喂食基础饲料直至7个月。在那时，将每组原始组（HF或HS）中的一半动物用HF饮食挑战3天；另一半喂食基础饲料。喂食基础饲料的HF组和HS组动物之间的血清胆固醇没有显著差异：HF组，73±7mg/dl（n = 11）；HS组，80±19（6）；P大于0.25。最初喂食HF饮食的动物在后期接受HF挑战后血清胆固醇显著升高（140±31mg/dl，n = 10，与喂食基础饲料相比P小于0.001）；最初喂食HS饮食的组则没有（93±7mg/dl，n = 5，P大于0.19）。即使没有胆固醇，早期接触HF饮食，在成年期短暂接触HF饮食挑战后也会引发高胆固醇血症反应。
