在卵巢灌注过程中对体外排卵的兔卵进行体外受精。

In-vitro fertilization of rabbit ova ovulated in vitro during ovarian perfusion.

作者信息

Kobayashi Y, Santulli R, Wright K H, Wallach E E

出版信息

J Reprod Fertil. 1983 May;68(1):41-4. doi: 10.1530/jrf.0.0680041.

DOI:10.1530/jrf.0.0680041

Abstract

During in-vitro perfusion of rabbit ovaries, ova that ovulated in response to hCG were recovered and cultured with spermatozoa capacitated in vivo. After culture for 30 h, 113 (48.8%) of the ova underwent cleavage and were not degenerate. Of these 113 zygotes, 75 were successfully transferred to the oviducts of 14 gonadotrophin-treated host rabbits. On Day 14 after transfer there were 14 implantation sites and 9 pregnancies proceeded to term. These data indicate that in-vitro ovulated ova can be fertilized in vitro and be used to establish normal pregnancies in a host rabbit.

摘要

在兔卵巢的体外灌注过程中，收集对人绒毛膜促性腺激素（hCG）有反应而排卵的卵子，并与体内获能的精子一起培养。培养30小时后，113个（48.8%）卵子发生分裂且未退化。在这113个受精卵中，75个成功移植到14只经促性腺激素处理的受体兔的输卵管中。移植后第14天，有14个着床部位，9只怀孕母兔顺利分娩。这些数据表明，体外排卵的卵子能够在体外受精，并用于在受体兔体内建立正常妊娠。