Lubinsky-Mink S, Munkenbeck P, Morrison D C
J Reticuloendothel Soc. 1983 May;33(5):353-67.
Insolubilized lipopolysaccharides (LPS) were prepared by covalently coupling LPS from polysaccharide-deficient S. minnesota R595 and polysaccharide-rich E. coli 055:B5 to carboxylated latex particles. The stability of these LPS-latex complexes was determined using several assays to detect soluble LPS following incubation at ambient and elevated temperatures. Resident and thioglycollate-elicited macrophages from both LPS-responder C3HeB/FeJ and LPS nonresponder C3H/HeJ mice were examined for their capacity to phagocytose the LPS particles following in vitro culture for various time periods. Uptake was demonstrated by an increase in the number of particles within the macrophages with increasing time of incubation. Rough polysaccharide-deficient LPS-latex particles were found to be more readily phagocytosed than control particles, whereas smooth polysaccharide-rich LPS particles were phagocytosed less readily than the controls. Qualitatively similar results were found in the relative rate of uptake of particles by the macrophages from the endotoxin-responsive and -unresponsive mouse strains used in this study.
通过将来自多糖缺陷型明尼苏达沙门氏菌R595和富含多糖的大肠杆菌055:B5的脂多糖（LPS）共价偶联到羧化乳胶颗粒上，制备了不溶性LPS。使用几种测定法来检测在环境温度和升高温度下孵育后可溶性LPS，从而确定这些LPS-乳胶复合物的稳定性。对来自LPS反应性C3HeB/FeJ小鼠和LPS无反应性C3H/HeJ小鼠的驻留巨噬细胞和巯基乙酸盐诱导的巨噬细胞在体外培养不同时间段后吞噬LPS颗粒的能力进行了检测。随着孵育时间的增加，巨噬细胞内颗粒数量的增加证明了摄取的发生。发现粗糙的多糖缺陷型LPS-乳胶颗粒比对照颗粒更容易被吞噬，而光滑的富含多糖的LPS颗粒比对照颗粒更不容易被吞噬。在本研究中使用的内毒素反应性和无反应性小鼠品系的巨噬细胞摄取颗粒的相对速率方面，发现了定性相似的结果。
