恶性高热的研究：通过二维凝胶电泳分析正常猪和氟烷敏感猪的骨骼肌蛋白质
Investigation of malignant hyperthermia: analysis of skeletal muscle proteins from normal and halothane sensitive pigs by two dimensional gel electrophoresis.
作者信息
Lorkin P A, Lehmann H
出版信息
J Med Genet. 1983 Feb;20(1):18-24. doi: 10.1136/jmg.20.1.18.
Abstract
Two dimensional gel analysis of skeletal muscles from normal pigs and from pigs which were homozygous for halothane sensitivity showed no obvious differences in the patterns of spots attributed to the major contractile proteins and glycolytic enzymes. In muscle from a sensitive pig which died of heat shock under anaesthesia there was a selective loss of glyceraldehyde-3-phosphate dehydrogenase and aldolase, presumably owing to proteolytic activity. The progressive loss of these enzymes under anaesthesia could contribute to the mechanism of heat production by diverting fructose 1,6 diphosphate into a futile cycle.
摘要
对正常猪以及对氟烷敏感的纯合子猪的骨骼肌进行二维凝胶分析，结果显示，主要收缩蛋白和糖酵解酶的斑点图谱并无明显差异。在一头在麻醉状态下死于热休克的敏感猪的肌肉中，3-磷酸甘油醛脱氢酶和醛缩酶出现了选择性缺失，推测这是蛋白水解活性所致。麻醉状态下这些酶的逐渐缺失，可能会通过将1,6-二磷酸果糖导入无效循环，从而促成产热机制。
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