Held I R
J Neurosci Res. 1983;9(2):203-10. doi: 10.1002/jnr.490090212.
The slow-twitch soleus muscle of the rat hindlimb was denervated by cutting the sciatic nerve in the midthigh on one side. A sham operation was performed on the contralateral side to provide a control soleus muscle. At 24, 48, 72, and 120 hours after these surgical procedures, the animals were sacrificed and the nuclei which were isolated from these muscles incubated in a phosphorylating medium containing [gamma-32P]ATP. At the 48 hour denervation period only, the in vitro phosphorylation of TCA-precipitable nuclear proteins was significantly increased compared to control values. Resolution of the SDS-solubilized nuclear proteins by polyacrylamide gel electrophoresis revealed that the increased phosphorylation was more marked in some phosphoproteins than others. The significance of these early denervation changes are discussed with respect to increases in the activities of the nuclear RNA polymerases and changes in the phosphorylation of cytosolic protein.
通过切断一侧大鼠大腿中部的坐骨神经，使其后肢的慢肌比目鱼肌失神经支配。对另一侧进行假手术，以提供对照比目鱼肌。在这些手术操作后的24、48、72和120小时，处死动物，从这些肌肉中分离出细胞核，并在含有[γ-32P]ATP的磷酸化培养基中孵育。仅在失神经支配48小时时，与对照值相比，三羧酸循环（TCA）沉淀的核蛋白的体外磷酸化显著增加。通过聚丙烯酰胺凝胶电泳解析十二烷基硫酸钠（SDS）溶解的核蛋白，结果显示，某些磷蛋白的磷酸化增加比其他磷蛋白更明显。结合核RNA聚合酶活性的增加和胞质蛋白磷酸化的变化，讨论了这些早期失神经支配变化的意义。
