Yeung C H, Woolley D M
J Submicrosc Cytol. 1983 Jan;15(1):327-31.
Mature hamster sperm were demembranated with Triton X-100, and the principal piece of the flagellum was reactivated by iontophoretic application of ATP. The response was a brief local straightening of a short length of the flagellum followed by the formation of a pair of bends at the two ends of the straight region. The formation of a PR (principal and reverse bends in a proximo-distal sequence) or an RP bend-pair is interpreted as a result of active sliding of doublet subset (1-4) or (6-9) respectively in the interbend region. The percentage of PR responses increased with the initial local curvature of the resting R bend and the distance of the stimulated site from the flagellar base, and decreased with the age of the preparation.
成熟的仓鼠精子用Triton X-100去膜，鞭毛的主段通过离子电渗法施加ATP而被重新激活。反应是鞭毛一小段的短暂局部伸直，随后在伸直区域的两端形成一对弯曲。PR（近端到远端序列中的主弯曲和反向弯曲）或RP弯曲对的形成分别被解释为双联体亚组（1-4）或（6-9）在弯曲间区域主动滑动的结果。PR反应的百分比随着静止R弯曲的初始局部曲率以及刺激部位离鞭毛基部的距离增加而增加，并随着标本的老化而降低。
