Ma Y P, Leung T M, Lau S H, Kwan H C
Microvasc Res. 1983 Mar;25(2):133-44. doi: 10.1016/0026-2862(83)90010-9.
The present article describes a new method of microvessel dimensions measurement in which a writing oscilloscope is used to continuously and graphically record the microvessel video signal from television microscopy at a fast rate of 50 records per second. Dimensions of interest, such as the microvessel red blood cell flux diameter, are then easily marked out manually from the graphic records and a dynamic electrical signal proportional to the dimensions is generated. The signal is then recorded on one channel of a multichannel voltage recorder and is synchronised with other experimental signals which have previously been recorded on-line during the experiment. The result is that the dimension signal appears to have been recorded on-line during the experiment as well. This is desirable for electronic signal correlation and processing. This method is useful when poor experimental conditions, commonly encountered, make automatic recording of microvessel dimension unsatisfactory and manual inspection and processing become necessary.
本文介绍了一种测量微血管尺寸的新方法,该方法使用书写示波器以每秒50次记录的快速速率连续地、以图形方式记录来自电视显微镜的微血管视频信号。然后,从图形记录中很容易手动标记出感兴趣的尺寸,例如微血管红细胞通量直径,并生成与该尺寸成比例的动态电信号。该信号随后被记录在多通道电压记录仪的一个通道上,并与实验期间先前已在线记录的其他实验信号同步。结果是,尺寸信号在实验期间似乎也已在线记录。这对于电子信号的关联和处理是很有必要的。当通常遇到的恶劣实验条件使得微血管尺寸的自动记录不尽人意且需要人工检查和处理时,这种方法很有用。