Ermakova G N, Nefelova M V, Egorov N S
Mikrobiologiia. 1983 Jan-Feb;52(1):109-13.
The fine structure of consecutive modifications was studied in sporulating cells of Bacillus polymyxa 153 growing in a liquid aerated medium. The stages of sporogenesis were found to be similar, in principle, with those in other species of the Bacillus genus, but also had certain peculiarities. In contrast to other bacilli, the nucleoid axial thread was not formed in B. polymyxa cells at the first stage of sporulation. The structure of the of the prospore nucleoid forming the local central zone differed from that of the vegetative cell nucleoid at the second stage. The ultrastructural changes observed when the spore septa was formed indicated that its structure was identical with that of the septa of vegetative division, in contrast to which the cell wall material did not undergo lysis but was preserved at the following stages of sporogenesis. The formation of the spore envelope material could easily be discerned in the cytoplasm of the mother cell. The fine structure of mature spores is described in this paper for B. polymyxa. The outer rigid part of its spore envelope was shown, for the first time, to consist of three layers, the surface one having an ordered fibrillar structure.
在通气液体培养基中生长的多粘芽孢杆菌153的产孢细胞中，研究了连续修饰的精细结构。发现孢子形成阶段原则上与芽孢杆菌属其他物种的阶段相似，但也有某些特点。与其他杆菌不同，在多粘芽孢杆菌细胞产孢的第一阶段未形成类核轴向丝。形成局部中央区的前孢子类核的结构在第二阶段与营养细胞核的结构不同。形成孢子隔膜时观察到的超微结构变化表明，其结构与营养分裂隔膜的结构相同，与之相反的是，细胞壁物质未发生溶解，而是在随后的产孢阶段保留下来。在母细胞的细胞质中很容易辨别出孢子包膜物质的形成。本文描述了多粘芽孢杆菌成熟孢子的精细结构。首次表明其孢子包膜的外部刚性部分由三层组成，最外层具有有序的纤维状结构。
