放射敏感型小鼠细胞突变体经X射线照射后DNA合成的抑制与恢复

Inhibition and recovery of DNA synthesis after X-irradiation in radiosensitive mouse-cell mutants.

作者信息

Hama-Inaba H, Hieda-Shiomi N, Sato K

出版信息

Mutat Res. 1983 May;120(2-3):161-5. doi: 10.1016/0165-7992(83)90158-6.

Abstract

The mouse lymphoma L5178Y cell line and its radiosensitive variants M10 and LX830 were examined for DNA synthesis after X-irradiation. The dose-response curves show that the rates of DNA synthesis immediately after exposure are reduced in a dose-dependent fashion and that the extents of reduction in these 3 cell lines are similar to one another. But a difference was observed in the recovery of DNA synthesis with time of incubation. The recovered levels in M10 and LX830 cells were much higher than those in L5178Y cells at high doses of X-rays. These results are discussed in relation to radioresistant DNA synthesis in ataxia telangiectasia cells.

摘要

对小鼠淋巴瘤L5178Y细胞系及其辐射敏感变体M10和LX830进行了X射线照射后DNA合成的检测。剂量反应曲线表明，照射后立即进行的DNA合成速率呈剂量依赖性降低，且这三种细胞系的降低程度彼此相似。但随着孵育时间的延长，在DNA合成的恢复方面观察到了差异。在高剂量X射线照射下，M10和LX830细胞中DNA合成的恢复水平远高于L5178Y细胞。结合共济失调毛细血管扩张症细胞中的抗辐射DNA合成对这些结果进行了讨论。