Plowman G D, Brown J P, Enns C A, Schröder J, Nikinmaa B, Sussman H H, Hellström K E, Hellström I
Nature. 1983;303(5912):70-2. doi: 10.1038/303070a0.
p97 is a 97,000 molecular weight cell-surface glycoprotein, which is present in human melanomas but in only trace amounts in normal adult tissues. Amino acid sequence and iron binding studies have shown that p97 is structurally and functionally related to transferrin. Reports that the genes for the transferrin receptor (TR) and possibly transferrin are located on chromosome 3 prompted us to investigate the chromosomal localization of the p97 gene. Our strategy was to characterize interspecies somatic cell hybrids derived from human fibroblasts or lymphocytes for expression of p97 and presence of human chromosomes. Although fibroblasts and lymphocytes express only small amounts of p97, we were able to type the hybrids for p97 by using monoclonal antibodies in highly sensitive and specific immunoassays. Of 14 hybrids, 6 contained chromosome 3 and expressed p97, and 8 were negative for both. We conclude that the p97 gene, like the transferrin and TR genes, is located on chromosome 3.
p97是一种分子量为97,000的细胞表面糖蛋白，存在于人类黑色素瘤中，但在正常成人组织中仅微量存在。氨基酸序列和铁结合研究表明，p97在结构和功能上与转铁蛋白相关。有报道称转铁蛋白受体（TR）基因以及可能的转铁蛋白基因位于3号染色体上，这促使我们研究p97基因的染色体定位。我们的策略是鉴定源自人成纤维细胞或淋巴细胞的种间体细胞杂种，以检测p97的表达和人类染色体的存在情况。尽管成纤维细胞和淋巴细胞仅表达少量的p97，但我们能够通过在高度敏感和特异的免疫测定中使用单克隆抗体对杂种进行p97分型。在14个杂种中，6个含有3号染色体并表达p97，8个两者均为阴性。我们得出结论，p97基因与转铁蛋白和TR基因一样，位于3号染色体上。
